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AB9255

Anti-RFX5 antibody

5

(1 Review)

|

(4 Publications)

Rabbit Polyclonal RFX5 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DNA-binding protein RFX5 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DNA-binding protein RFX5, Regulatory factor X 5, RFX5

1 Images
Western blot - Anti-RFX5 antibody (AB9255)
  • WB

Unknown

Western blot - Anti-RFX5 antibody (AB9255)

ab9255 at a 1 : 1000 dilution staining RFX5 in 500μg of HeLa Nuclear Extract by Western Blot (ECL). Immunoprecipitation was performed prior to Western Blotting with 2.0μg of ab9255. ab9255 at a 1 : 1000 dilution staining RFX5 in 500µg of HeLa Nuclear Extract by Western Blot (ECL). Immunoprecipitation was performed prior to Western Blotting with 2.0µg of ab9255.

All lanes:

Western blot - Anti-RFX5 antibody (ab9255)

Predicted band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DNA-binding protein RFX5 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P48382

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFX5 also known as Regulatory Factor X 5 is a transcription factor characterized by a mass of approximately 74 kDa. It plays an essential role in the modulation and transcriptional regulation of genes. RFX5 is predominantly expressed in the nucleus of human cells where it binds to DNA and regulates gene expression. This protein is vital to the regulation of major histocompatibility complex (MHC) class II genes affecting how cells present antigens.
Biological function summary

RFX5 functions as part of the RFX complex an important element for the transcription of MHC class II genes. It interacts with other regulatory factor X proteins to form this complex enabling the activation of target gene transcription. RFX5 acts by binding to the X box motifs within the promoters of the MHC class II genes. Its presence is necessary to maintain the immune response capability by ensuring the expression of these genes which is a fundamental process in antigen presentation to immune cells.

Pathways

RFX5 integrates into the MHC class II antigen presentation pathway. It has a significant relationship with the CIITA transcriptional regulator with CIITA enhancing the activity of RFX5 during the assembly of the MHC class II transcriptional machinery. Another important pathway is the immune response pathway where RFX5 is important in coordinating the expression of genes that help the immune system recognize and respond to pathogens effectively.

Deficiencies or malfunctions in RFX5 have strong connections with Bare Lymphocyte Syndrome (BLS) type II marked by reduced expression of MHC class II molecules. In this context RFX5's interactions with CIITA are critical because improper function of either can lead to compromised immune responses. Studies also suggest an association between disrupted RFX5 activity and autoimmune diseases where improper antigen presentation can lead to the immune system attacking the body's own tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
See full target information DNA-binding protein RFX5
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:349 PubMed33824282

2021

ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Huojian Shen,Hongyi Zhu,Yuanwen Chen,Zhiyong Shen,Weiqing Qiu,Changlin Qian,Jie Zhang

Circulation 138:1850-1863 PubMed29794082

2018

Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Paul A Mueller,Lin Zhu,Hagai Tavori,Katherine Huynh,Ilaria Giunzioni,John M Stafford,MacRae F Linton,Sergio Fazio

Oncotarget 8:93878-93898 PubMed29212196

2017

Juglanin inhibits lung cancer by regulation of apoptosis, ROS and autophagy induction.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Ya-Qiong Xiong,Jing Xu,Ji-Peng Wang,Zi-Li Meng,Yong-Qing Hong

Molecular microbiology 61:1543-55 PubMed16968227

2006

Treatment of Chlamydia trachomatis with a small molecule inhibitor of the Yersinia type III secretion system disrupts progression of the chlamydial developmental cycle.

Applications

Unspecified application

Species

Unspecified reactive species

K Wolf,H J Betts,B Chellas-Géry,S Hower,C N Linton,K A Fields
View all publications
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