AB103497

Anti-RFX6 antibody

Name: Anti-RFX6 antibody (ab103497) | Abcam
Brand: Abcam Limited
SKU: AB103497

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(1 Publication)

Rabbit Polyclonal RFX6 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RFX6.

View Alternative Names

RFXDC1, RFX6, DNA-binding protein RFX6, Regulatory factor X 6, Regulatory factor X domain-containing protein 1

2 Images

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Western blot - Anti-RFX6 antibody (AB103497)

All lanes:

Western blot - Anti-RFX6 antibody (ab103497) at 1/500 dilution

All lanes:

Human kidney lysate at 50 µg

Predicted band size: 101 kDa,102 kDa,103 kDa,104 kDa,105 kDa,106 kDa,107 kDa,108 kDa,109 kDa,110 kDa,111 kDa,112 kDa,113 kDa,114 kDa,116 kDa,117 kDa,118 kDa,121 kDa,124 kDa,125 kDa,128 kDa,129 kDa,132 kDa,134 kDa,136 kDa,138 kDa,142 kDa,143 kDa,148 kDa,153 kDa,192 kDa,76 kDa,79 kDa,80 kDa,81 kDa,82 kDa,85 kDa,86 kDa,87 kDa,88 kDa,89 kDa,90 kDa,91 kDa,93 kDa,94 kDa,96 kDa,97 kDa,98 kDa,99 kDa

Observed band size: 100 kDa,104 kDa,105 kDa,110 kDa,112 kDa,114 kDa,115 kDa,120 kDa,125 kDa,130 kDa,136 kDa,140 kDa,143 kDa,25 kDa,28 kDa,37 kDa,45 kDa,50 kDa,65 kDa,82 kDa,85 kDa,90 kDa,91 kDa,97 kDa,98 kDa,99 kDa

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Western blot - Anti-RFX6 antibody (AB103497)

All lanes:

Western blot - Anti-RFX6 antibody (ab103497) at 1/500 dilution

Lane 1:

RFX6 transfected 293T cell line lysate at 25 µg

Lane 2:

Non transfected 293T cell line lysate at 25 µg

Predicted band size: 102 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RFX6.

Q8HWS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFX6 also known as Regulatory Factor X6 is a transcription factor with a molecular weight of approximately 102 kDa. It belongs to the RFX family of transcription factors which are critical in regulating gene expression. RFX6 is mainly expressed in the pancreas particularly in the islets of Langerhans as well as in developing gut tissues. This protein contains a well-conserved DNA-binding domain and functions as an important regulator of insulin production and secretion.

Biological function summary

RFX6 plays a significant role in the differentiation and development of pancreatic beta-cells which are essential for insulin production. RFX6 acts as part of a larger transcriptional complex working in conjunction with other transcription factors to ensure proper gene expression during the development of insulin-secreting cells. Its activity is pivotal in maintaining glucose homeostasis and proper metabolic function.

Pathways

RFX6 plays an essential role in the Wnt signaling pathway which influences the development of both pancreatic and intestinal cells. In this context RFX6 works closely with proteins like TCF7L2 another transcription factor active in the Wnt pathway. RFX6 also interacts with the Notch signaling pathway further highlighting its importance in the differentiation of pancreatic endocrine cells alongside proteins like HES1.

Mutations or dysfunctions in RFX6 can lead to Mitchell-Riley syndrome and neonatal diabetes. Mitchell-Riley syndrome manifests with multiple developmental anomalies including pancreatic hypoplasia while neonatal diabetes results from impaired insulin production due to beta-cell dysfunction. These conditions illustrate the importance of RFX6 in maintaining proper functional development of the pancreas. In some contexts RFX6 interaction with proteins like INS the gene encoding insulin highlights its direct influence on insulin-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcription factor required to direct islet cell differentiation during endocrine pancreas development. Specifically required for the differentiation of 4 of the 5 islet cell types and for the production of insulin (PubMed : 20148032, PubMed : 25497100). Not required for pancreatic PP (polypeptide-producing) cells differentiation. Acts downstream of NEUROG3 and regulates the transcription factors involved in beta-cell maturation and function, thereby restricting the expression of the beta-cell differentiation and specification genes, and thus the beta-cell fate choice. Activates transcription by forming a heterodimer with RFX3 and binding to the X-box in the promoter of target genes (PubMed : 20148032). Involved in glucose-stimulated insulin secretion by promoting insulin and L-type calcium channel gene transcription (PubMed : 25497100).

See full target information RFX6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 35:50-8 PubMed26530530

2015

Latexin exhibits tumor-suppressor potential in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanxiong Xue,Yuhui Zhou,Cheng Wang,Jihang Zheng,Pu Zhang,Lingling Zhou,Liang Wu,Yunfeng Shan,Mengsi Ye,Yun He,Zhenzhai Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RFX6 antibody

Western blot - Anti-RFX6 antibody (AB103497)

Western blot - Anti-RFX6 antibody (AB103497)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise