AB236408

Anti-RFXANK antibody [OTI3E7]

Name: Anti-RFXANK antibody [OTI3E7] (ab236408) | Abcam
Brand: Abcam Limited
SKU: AB236408
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal RFXANK antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RFXANK.

View Alternative Names

ANKRA1, RFXB, RFXANK, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFX-B

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human pancreas tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human tonsil tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human kidney carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human ovary adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human bladder carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human kidney tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (AB236408)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (AB236408)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY RFXANK stained for RFXANK (green) using ab236408 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-RFXANK antibody [OTI3E7] (AB236408)

All lanes:

Western blot - Anti-RFXANK antibody [OTI3E7] (ab236408) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY RFXANK cDNA transfected HEK-293T cell lysate at 5 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3E7

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RFXANK.

O14593

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Clone OTI3E7 (formerly 3E7).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RFXANK protein also known as regulatory factor X-associated ankyrin-containing protein is a component of the RFX complex. RFXANK has a molecular mass of approximately 27 kDa. This protein plays a mechanical role in the regulation of transcription by interacting with DNA sequences and recruiting various co-factors necessary for gene expression. RFXANK is largely expressed in immune cells where it modulates processes important for immune function.

Biological function summary

RFXANK acts as part of the RFX complex which is known for its role in the regulation of class II major histocompatibility complex (MHC) genes. These genes are significant for proper immune response allowing immune cells to present antigens and initiate defense mechanisms. RFXANK through its interaction with other proteins like RFX5 and RFXAP stabilizes the DNA binding ability of the RFX complex enabling the transcription of genes essential for antigen presentation.

Pathways

The regulatory influence of RFXANK integrates into important immune pathways such as the MHC class II antigen presentation pathway. RFXANK's partnership with RFX5 and RFXAP facilitates binding to the X-box motif of MHC class II promoters ensuring the transcription initiation required for effective immune surveillance. These relations evidence RFXANK's vital place in immune system operations particularly in adaptive immunity.

RFXANK mutations or dysregulation relate to bare lymphocyte syndrome type II a severe immunodeficiency disorder. This condition arises when RFXANK fails to properly regulate MHC class II expression compromising antigen presentation and adaptive immune response. Connections exist between RFXANK and other disease-related proteins like RFX5 which highlight disruptions at the molecular level impacting immune functionality.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed : 10072068, PubMed : 10725724, PubMed : 9806546). May also potentiate the activation of RAF1 (By similarity).. Isoform 2 is not involved in the positive regulation of MHC class II genes.

See full target information RFXANK

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of digestive diseases 23:376-387 PubMed35844201

2022

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Rong Lin,Wei Rong Chen,Pei Hong Zheng,Wei Shan Chen,Gao Yang Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFXANK antibody [OTI3E7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (AB236408)

Western blot - Anti-RFXANK antibody [OTI3E7] (AB236408)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise