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AB51376

Anti-RFXAP antibody [RFXAD55A]

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(1 Publication)

Mouse Monoclonal RFXAP antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RFXAP aa 50-200.

View Alternative Names

Regulatory factor X-associated protein, RFX-associated protein, RFX DNA-binding complex 36 kDa subunit, RFXAP

1 Images
Western blot - Anti-RFXAP antibody [RFXAD55A] (AB51376)
  • WB

Unknown

Western blot - Anti-RFXAP antibody [RFXAD55A] (AB51376)

All lanes:

Western blot - Anti-RFXAP antibody [RFXAD55A] (ab51376) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

Anti mouse IgG antibody at 1/2500 dilution

Predicted band size: 28 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RFXAD55A

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RFXAP aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

O00287

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Filtered through a 0.22 µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RFXAP also known as Regulatory Factor X Associated Protein plays a role as a component in the transcriptional activation of MHC class II genes. This protein modulates gene expression by forming a complex with other transcription factors to bind to specific sequences in the MHC class II promoter region. It has a molecular mass of approximately 31 kDa. RFXAP is ubiquitously expressed in various tissues demonstrating a broad pattern that supports its fundamental function in cellular processes involved with immune response.
Biological function summary

RFXAP functions as an integral part of the RFX complex which also includes RFX5 and RFXANK. This complex acts as a transcriptional activator for MHC class II genes necessary for antigen presentation to CD4+ T lymphocytes. By participating in the assembly of the enhanceosome on the SXY module of MHC class II promoters RFXAP ensures regulated and proper transcription of these genes affecting immune system efficiency and adaptability.

Pathways

RFXAP contributes directly to the MHC class II expression pathway essential for adaptive immunity. It interacts and works closely with RFX5 and RFXANK within the same regulatory complex facilitating the transcription of major histocompatibility complex class II molecules. Proper function of these molecules is necessary for the recognition and processing of foreign antigens affecting how the immune system responds to pathogens.

Mutations or functional disruptions of RFXAP are linked to Bare Lymphocyte Syndrome Type II a condition characterized by the absence of MHC class II expression on antigen-presenting cells leading to severe immunodeficiency. In this disorder the connection with other proteins like RFX5 and RFXANK is critical since defects in any of these components can result in similar pathological outcomes highlighting the importance of RFXAP within the MHC class II transcriptional machinery.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the RFX complex that binds to the X-box of MHC II promoters.
See full target information RFXAP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 284:6955-65 PubMed19131340

2009

Atrophin proteins interact with the Fat1 cadherin and regulate migration and orientation in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Hou,Nicholas E S Sibinga
View all publications
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Product promise

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