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AB127687

Anti-RG9MTD2 antibody

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(1 Publication)

Rabbit Polyclonal RG9MTD2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRMT10A aa 50-300.

View Alternative Names

RG9MTD2, TRMT10A, tRNA methyltransferase 10 homolog A, RNA (guanine-9-)-methyltransferase domain-containing protein 2, tRNA (guanine(9)-N(1))-methyltransferase TRMT10A

1 Images
Western blot - Anti-RG9MTD2 antibody (AB127687)
  • WB

Unknown

Western blot - Anti-RG9MTD2 antibody (AB127687)

10% SDS PAGE

All lanes:

Western blot - Anti-RG9MTD2 antibody (ab127687) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TRMT10A aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q8TBZ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RG9MTD2 also known as tRNA (guanine-N(7)-)-methyltransferase is an enzyme involved in modifying guanine bases at the N7 position in tRNA molecules. This enzyme has a molecular mass of approximately 25 kDa. It is mostly expressed in the cytoplasm where it participates in the post-transcriptional modification of tRNA a critical step for proper protein translation and function.
Biological function summary

The enzyme functions by catalyzing the methyl transfer from S-adenosylmethionine (SAM) to guanine in tRNA assisting in the stabilizing of tRNA structure and enhancing its functional efficiency. RG9MTD2 acts within a complex network of other enzymes that ensure proper tRNA maturation. By influencing the structural integrity of tRNA RG9MTD2 plays an essential role in maintaining cellular homeostasis and protein synthesis fidelity.

Pathways

The enzyme is actively involved in the methylation pathway that modifies tRNA molecules an important process in the RNA processing pathway. It interacts with other tRNA-modifying enzymes like TRMT10C and NSUN2 which participate in the regulation of tRNA structure and function. RG9MTD2's activity closely ties into the broader cellular pathways like translation and protein biosynthesis ensuring the accurate expression of genetic information.

RG9MTD2 mutations or dysregulation can relate to tRNA-associated disorders such as some forms of mitochondrial encephalomyopathy. The enzyme's role in maintaining tRNA stability can affect proteins involved in other disorders linking it to a broader understanding of genetic diseases. Variants in genes encoding tRNA methyltransferases have associations with neurological disorders highlighting the importance of RG9MTD2 in the context of genetic stability and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs (PubMed : 23042678, PubMed : 25053765). Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs (PubMed : 23042678).
See full target information TRMT10A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical science (London, England : 1979) 133:2361-2378 PubMed31763675

2019

DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Heng-Huei Lin,Tzong-Shyuan Lee,Shing-Jong Lin,Yi-Chen Yeh,Tse-Min Lu,Chiao-Po Hsu
View all publications
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Product promise

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