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AB151975

Anti-RG9MTD3 antibody

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(1 Publication)

Rabbit Polyclonal RG9MTD3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRMT10B aa 50 to C-terminus.

View Alternative Names

RG9MTD3, TRMT10B, tRNA methyltransferase 10 homolog B, RNA (guanine-9-)-methyltransferase domain-containing protein 3, tRNA (guanine(9)-N(1))-methyltransferase TRMT10B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RG9MTD3 antibody (AB151975)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RG9MTD3 antibody (AB151975)

Immunohistochemical analysis of paraffin embedded Human A549 xenograft tissue labeling RG9MTD3 with ab151975 at a 1/500 dilution.

Western blot - Anti-RG9MTD3 antibody (AB151975)
  • WB

Unknown

Western blot - Anti-RG9MTD3 antibody (AB151975)

10% SDS PAGE

All lanes:

Western blot - Anti-RG9MTD3 antibody (ab151975) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRMT10B aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6PF06

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RG9MTD3 also known as tRNA (guanine-N(7)-)-methyltransferase or WDR4 is an enzyme responsible for methylation at the guanine N7 position in tRNA molecules. It has a mass of approximately 40 kDa. RG9MTD3 is expressed in various human tissues including the liver brain and lungs. The precise modulation of tRNA has critical implications for protein synthesis influencing overall cellular function and protein quality.
Biological function summary

RG9MTD3 influences the modification of tRNA through its enzymatic action which is essential for efficient protein translation. It does not function in isolation but as part of a larger tRNA modification complex. The enzyme's role includes the fine-tuning of translation fidelity and efficiency impacting overall protein biosynthesis and cellular homeostasis. By ensuring correct tRNA modification RG9MTD3 influences translation accuracy which is significant for cellular processes.

Pathways

The activity of RG9MTD3 connects to the tRNA modification pathway and indirectly to the translation pathway where its actions influence protein synthesis. It shows interaction with other proteins involved in tRNA processing and protein translation such as MTT2. Through its integration in these pathways RG9MTD3 ensures that translation proceeds smoothly and that cells maintain proper functional proteomes.

RG9MTD3 has links to certain developmental disorders such as Galloway-Mowat syndrome. This syndrome characterized by developmental delays and cranial abnormalities connects with alterations in the function of proteins linked to tRNA modifications impacting the translation process. The function of RG9MTD3 also connects to broader metabolic disorders where its role in protein synthesis can be indirectly associated with metabolic enzymes affecting cellular metabolism and energy regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs (PubMed : 23042678). Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs (PubMed : 23042678).
See full target information TRMT10B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of pathology 258:106-120 PubMed35696251

2022

Functional genomics screening identifies aspartyl-tRNA synthetase as a novel prognostic marker and a therapeutic target for gastric cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Hiroto Katoh,Daisuke Komura,Asami Yamamoto,Mieko Ochi,Takumi Onoyama,Hiroyuki Abe,Tetsuo Ushiku,Yasuyuki Seto,Jian Suo,Shumpei Ishikawa
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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