Anti-RG9MTD3 antibody [EPR28590-15]

• WB

Supplier Data

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (AB324235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The band beneath the target band (40 kDa) in lanes 1 is likely to be a degraded target fragment.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (ab324235) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 40 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg

Lane 4:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (AB324235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (ab324235) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

HeLa transfected with siRNA specifically targeting RG9MTD3 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (AB324235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon, heart

The band beneath the target band (44 kDa) in lanes 1 is likely to be a degraded target fragment.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (ab324235) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 40 µg

Lane 2:

Mouse colon tissue lysate at 40 µg

Lane 3:

Mouse heart tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (AB324235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In lanes 1-3, the lysates were stored at -80℃ prior to Western Blotting. The bands beneath the target band (44kDa) are likey to be degradation products. In lanes 4-6, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

Lanes 6 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 125 seconds; Lanes 4-5 : 180 seconds; Lane 6 : 125 seconds

All lanes:

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (ab324235) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate (Frozen lysates) at 40 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Frozen lysates) at 40 µg

Lane 3:

Mouse testis tissue lysate (Frozen lysates) at 40 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Fresh lysates) at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate (Fresh lysates) at 20 µg

Lane 6:

Mouse testis tissue lysate (Fresh lysates) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (AB324235)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human RG9MTD1 and RG9MTD2

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RG9MTD3 antibody [EPR28590-15] (ab324235) at 1/1000 dilution

Lane 1:

His-tagged human RG9MTD2 recombinant protein at 10 ng

Lane 2:

His-tagged human RG9MTD3 recombinant protein at 10 ng

Lane 3:

His-tagged human RG9MTD1 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa,30 kDa

false

Exposure time: 1s