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AB254821

Anti-RGL3 antibody

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Rabbit Polyclonal RGL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ral guanine nucleotide dissociation stimulator-like 3 aa 100 to C-terminus.

View Alternative Names

Ral guanine nucleotide dissociation stimulator-like 3, RalGDS-like 3, RGL3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGL3 antibody (AB254821)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGL3 antibody (AB254821)

Paraffin-embedded human testis tissue stained for RGL3 using ab254821 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-RGL3 antibody (AB254821)
  • WB

Supplier Data

Western blot - Anti-RGL3 antibody (AB254821)

All lanes:

Western blot - Anti-RGL3 antibody (ab254821) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

RGL3 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 78 kDa

false

Western blot - Anti-RGL3 antibody (AB254821)
  • WB

Supplier Data

Western blot - Anti-RGL3 antibody (AB254821)

Lane 1:

Western blot - Anti-RGL3 antibody (ab254821)

Lane 2:

Western blot - Anti-RGL3 antibody (ab254821) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Ral guanine nucleotide dissociation stimulator-like 3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q3MIN7

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RGL3 also known as Ral guanine nucleotide dissociation stimulator-like 3 is a protein involved in regulating various cellular processes. It has a molecular weight of approximately 85 kDa. RGL3 is expressed in multiple tissues including the brain and liver indicating its involvement in diverse physiological functions. The protein interacts mechanically with other cellular proteins to promote GDP-GTP exchange on Ral GTPases which act as molecular switches in numerous signaling pathways.
Biological function summary

The protein RGL3 plays a role in cellular signaling and transformation. It is a part of the RalGDS protein family and functions within a complex that includes RalA and RalB GTPases. This complex is instrumental in directing cellular events that involve rearrangements in the cytoskeleton and membrane trafficking. RGL3 also impacts gene expression by modulating the activity of its associated GTPases which influence cell growth and differentiation.

Pathways

RGL3 is intricately linked to the Ras signaling pathway and the MAPK/ERK cascade. In the Ras pathway RGL3 associates with Ras proteins influencing cellular proliferation and survival. In the MAPK/ERK cascade RGL3 indirectly affects the downstream phosphorylating actions by modifying the activity of Ral GTPases thereby contributing to the regulation of mitogenic signals. These interactions reflect RGL3's significance in cellular signal transduction and communication.

Disruptions in RGL3 function have implications in certain cancers such as colorectal cancer where altered signaling dynamics lead to uncontrolled cell growth. RGL3's role in the Ras pathway links it to oncogenic transformation where mutations of Ras proteins result in aberrant signaling. Additionally there is evidence suggesting a relation to diabetes through its impact on insulin signaling pathways influencing glucose metabolism. The interplay between RGL3 and Ras proteins along with other molecules in these pathways highlights its potential impact on disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related protein M-Ras. Negatively regulates Elk-1-dependent gene induction downstream of HRas and MEKK1 (By similarity).
See full target information Ral guanine nucleotide dissociation stimulator-like 3
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Product promise

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For full details, please see our Terms & Conditions

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