AB157095

Anti-RGNEF antibody

Name: Anti-RGNEF antibody (ab157095) | Abcam
Brand: Abcam Limited
SKU: AB157095
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RGNEF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGEF28 aa 1650 to C-terminus.

View Alternative Names

KIAA1998, RGNEF, ARHGEF28, Rho guanine nucleotide exchange factor 28, 190 kDa guanine nucleotide exchange factor, Rho guanine nucleotide exchange factor, p190-RhoGEF, p190RhoGEF

2 Images

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Immunoprecipitation - Anti-RGNEF antibody (AB157095)

Immunoprecipitation. ab157095 at 1 µg/ml staining RGNEF in HeLa whole cell lysate immunoprecipitated using ab157095 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : Control IgG. Predicted band size : 192 kDa. Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-RGNEF antibody (ab157095)

Predicted band size: 192 kDa

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Western blot - Anti-RGNEF antibody (AB157095)

All lanes:

Western blot - Anti-RGNEF antibody (ab157095) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 192 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ARHGEF28 aa 1650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N1W1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RGNEF also known as Rho Guanine Nucleotide Exchange Factor functions as a signaling protein with a role in regulating the actin cytoskeleton and cellular architecture. It acts mechanistically by promoting the exchange of GDP for GTP on Rho proteins which are small GTPases involved in cytoskeletal dynamics. The protein has an approximate mass of 218 kDa. RGNEF is widely expressed in many tissues with higher levels found in the brain which suggests an importance in neural structure and function.

Biological function summary

RGNEF serves as a critical part of cell signaling processes that control morphogenesis motility and cell division. It acts within a multi-protein complex that includes other exchange factors and scaffold proteins coordinating its action and enhancing signal specificity. Its activity impacts various cellular functions often intersecting with pathways that regulate cytoskeletal reorganization and neural communication highlighting its importance in maintaining normal cell behavior.

Pathways

The functionality of RGNEF integrates into the Rho GTPase cycle and the MAPK signaling pathway. Through these pathways RGNEF interacts with proteins like RhoA Rac1 and Cdc42 which play pivotal roles in transmitting external signals that modulate the cytoskeleton and gene expression. These interactions help cells respond to environmental cues and sustain their structural integrity influencing developmental and physiological processes.

RGNEF is relevant to neurodegenerative diseases especially Amyotrophic Lateral Sclerosis (ALS) and cancer. Mutations and alterations in RGNEF are linked with the pathogenesis of ALS where its interaction with other proteins like TDP-43 contributes to neuronal dysfunction. In cancer dysregulation of RGNEF signaling is observed implicating its role in tumor progression and metastasis through its association with oncogenic proteins such as RhoA. Understanding the role of RGNEF in these conditions may offer insights into therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis (By similarity).

See full target information ARHGEF28

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 218:1725-1742 PubMed30948425

2019

VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa V Juettner,Kevin Kruse,Arkaprava Dan,Vinh H Vu,Yousaf Khan,Jonathan Le,Deborah Leckband,Yulia Komarova,Asrar B Malik

Molecular brain 10:46 PubMed28969660

2017

Novel miR-b2122 regulates several ALS-related RNA-binding proteins.

Applications

Species

Human

Zachary C E Hawley,Danae Campos-Melo,Michael J Strong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RGNEF antibody

Immunoprecipitation - Anti-RGNEF antibody (AB157095)

Western blot - Anti-RGNEF antibody (AB157095)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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