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AB325872

Anti-RGR antibody [HL3058] - BSA and Azide free

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Rabbit Recombinant Monoclonal RGR antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Transfected cell lysate - Human samples. Immunogen corresponding to Synthetic Peptide within Human RGR.

View Alternative Names

RPE-retinal G protein-coupled receptor, RGR

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGR antibody [HL3058] - BSA and Azide free (AB325872)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGR antibody [HL3058] - BSA and Azide free (AB325872)

ab325872 detects RGR protein by immunohistochemical analysis. Sample : Paraffin-embedded rat eye. Green : RGR stained by ab325872 diluted at 1 : 100. Red : beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGR antibody [HL3058] - BSA and Azide free (AB325872)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGR antibody [HL3058] - BSA and Azide free (AB325872)

ab325872 detects RGR protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse eye. Green : RGR stained by ab325872 diluted at 1 : 100. Red : beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-RGR antibody [HL3058] - BSA and Azide free (AB325872)
  • WB

Supplier Data

Western blot - Anti-RGR antibody [HL3058] - BSA and Azide free (AB325872)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ab325872 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-RGR antibody [HL3058] - BSA and Azide free (ab325872) at 1/5000 dilution

Lanes 1 and 3:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled RGR transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled RHO transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL3058

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RGR.

P47804

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for all-trans- and 11-cis-retinal. Binds preferentially to the former and may catalyze the isomerization of the chromophore by a retinochrome-like mechanism.
See full target information RGR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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