AB117077

Anti-RGS1 antibody

Name: Anti-RGS1 antibody (ab117077) | Abcam
Brand: Abcam Limited
SKU: AB117077

(1 Review)

(6 Publications)

Goat Polyclonal RGS1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RGS1 aa 150 to C-terminus.

View Alternative Names

1R20, BL34, IER1, RGS1, Regulator of G-protein signaling 1, B-cell activation protein BL34, Early response protein 1R20

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (AB117077)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (AB117077)

Human Small Intestine (formalin-fixed, paraffin-embedded) stained with ab117077 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (AB117077)

Human Thymus (formalin-fixed, paraffin-embedded) stained with ab117077 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-RGS1 antibody (AB117077)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.

All lanes:

Western blot - Anti-RGS1 antibody (ab117077) at 1 µg/mL

All lanes:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 24 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RGS1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Regulator of G-protein signaling 1 (RGS1) also known as BL34 or 1R20 acts mechanically as a GTPase-activating protein (GAP) for Gα subunits of heterotrimeric G-proteins. This function speeds up the deactivation of G-proteins by converting GTP to GDP effectively stopping signal transduction. RGS1 has a molecular mass of approximately 25 kDa. This protein is mainly expressed in B and T lymphocytes and you can find it in lymphoid tissues such as the spleen and lymph nodes.

Biological function summary

The RGS1 protein modulates immune cell signaling thereby controlling cell migration and positioning. RGS1 plays a role in reducing signaling from chemokine receptors which affects the movement of lymphocytes within tissues. It does not significantly form part of a larger complex mostly acting independently to exert its function within the immune system.

Pathways

The regulatory activity of RGS1 heavily influences the chemokine signaling pathway and the G-protein coupled receptor (GPCR) signaling pathway. These pathways are important for the directional migration and activation of immune cells. Within these pathways RGS1 interacts with related proteins such as CXCR4 and Gα subunits modulating their signaling outputs to regulate immune cell responses.

RGS1 links to autoimmune diseases and certain cancers. It can modulate the immune response in diseases such as multiple sclerosis where its activity may affect immune cell movement and function. Connections also exist between RGS1 and CXCR4 within cancer pathology where altered signaling could contribute to tumor growth and metastasis. By affecting these pathways RGS1 becomes a potential target for therapeutic intervention in related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the N-formylpeptide chemoattractant receptors and leukotriene receptors (PubMed : 10480894). Inhibits B cell chemotaxis toward CXCL12 (By similarity). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form (PubMed : 10480894, PubMed : 18434541).

See full target information RGS1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:864 PubMed39757280

2025

Regulator of G protein signaling-1 facilitates ovarian cancer development by modulating NF-kB signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Shengnan,Xi Jiayan,Sun Meng,Li Li,Cai Shengyun,Xu Mingjuan

Annals of translational medicine 10:982 PubMed36267780

2022

Regulator of G-protein signaling 1 promotes choroidal neovascularization in age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Fengbin Zhang,Yangchen Guo,Yanyan Liu,Ningxin Pan,Hong Chen,Ju Huang,Bifan Yu,Aimin Sang

Neuropsychiatric disease and treatment 17:1165-1181 PubMed33911865

2021

Biliverdin Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating the miR-27a-3p/Rgs1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Li,Lijia Peng,Wenya Bai,Peihua Peng,Wendong Chen,Wei Yang,Jianlin Shao

American journal of physiology. Regulatory, integrative and comparative physiology 320:R236-R249 PubMed33206556

2020

Signaling in rat brainstem via Gpr160 is required for the anorexigenic and antidipsogenic actions of cocaine- and amphetamine-regulated transcript peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Haddock,Gislaine Almeida-Pereira,Lauren M Stein,Matthew R Hayes,Grant R Kolar,Willis K Samson,Gina L C Yosten

Journal of cellular physiology 234:20432-20442 PubMed31012109

2019

RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll-like receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xumin Hu,Jianhua Tang,Gang Zeng,Xuyun Hu,Peng Bao,Jionglin Wu,Yuwei Liang,Weixi Deng,Yong Tang

International journal of oncology 54:1317-1326 PubMed30720136

2019

Long non-coding RNA TUG1 recruits miR‑29c‑3p from its target gene RGS1 to promote proliferation and metastasis of melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqian Wang,Gang Liu,Lin Ren,Kun Wang,Aimin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RGS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (AB117077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS1 antibody (AB117077)

Western blot - Anti-RGS1 antibody (AB117077)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise