JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249072

Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal RGS10 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

Regulator of G-protein signaling 10, RGS10

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)

This data was developed using ab154172, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of K562 cells labeling RGS10 with ab154172 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)

This data was developed using ab154172, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling RGS10 with ab154172 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)

This data was developed using ab154172, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154172 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)

This data was developed using ab154172, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154172 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)
  • WB

Unknown

Western blot - Anti-RGS10 antibody [EPR10657(B)] - BSA and Azide free (AB249072)

This data was developed using ab154172, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RGS10 antibody [EPR10657(B)] (<a href='/en-us/products/primary-antibodies/rgs10-antibody-epr10657b-ab154172'>ab154172</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10657(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab249072 is the carrier-free version of ab154172.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RGS10 also known as Regulator of G-protein Signaling 10 is a 20 kDa protein that regulates G-protein signaling. This protein accelerates the GTPase activity of the G alpha subunits leading to termination of the G-protein signaling. RGS10 expresses mainly in the brain and immune cells where it modulates signal transduction processes. It influences cellular responses significantly due to its ability to deactivate active G-proteins by increasing their GTPase activity.
Biological function summary

RGS10 plays a role in immune responses and neural function. It is not typically part of larger protein complexes but it interacts independently with other proteins in cellular signaling pathways. In the immune system RGS10 influences various processes such as cytokine production and leukocyte function. In neurons RGS10 contributes to maintaining neural signaling by regulating neurotransmitter-related G-protein coupled receptor pathways.

Pathways

G-protein signaling pathways and the MAPK signaling pathway include RGS10 as an important regulatory protein. It modulates the G-protein coupled receptor signaling pathway an important aspect of cellular response to external stimuli. RGS10 also influences the PI3K-Akt signaling pathway by regulating upstream signaling events. These pathways involve proteins such as G alpha subunits and PI3 kinase which interact with RGS10 to control diverse physiological processes.

Individuals with altered RGS10 expression may have neurodegenerative diseases and inflammatory disorders. Alzheimer's disease is one condition where RGS10's regulatory control affects neuronal cell survival and tau phosphorylation a process implicated in disease progression. Regarding inflammation its interaction with NLRP3 inflammasome proteins connects RGS10 to inflammatory pathways which can lead to disorders like rheumatoid arthritis. Adjusting RGS10 levels might modulate disease mechanisms highlighting its potential therapeutic interest.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed : 10608901, PubMed : 11443111, PubMed : 18434541, PubMed : 8774883, PubMed : 9353196). Modulates the activity of potassium channels that are activated in response to CHRM2 signaling (PubMed : 11443111). Activity on GNAZ is inhibited by palmitoylation of the G-protein (PubMed : 9353196).
See full target information RGS10
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB155285

Anti-UBC4 antibody [EPR11032(B)]

RabMAb|Recombinant

Western blot - Anti-UBC4 antibody [EPR11032(B)] (AB155285)

  • 0
  • 0
Primary Antibodies

AB154170

Anti-USP5 antibody [EPR10454]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170)

  • 0
  • 0
Primary Antibodies

AB248755

Anti-PEX7 antibody [EPR7714(2)(B)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-PEX7 antibody [EPR7714(2)(B)] - BSA and Azide free (AB248755)

  • 0
  • 0
Primary Antibodies

AB171084

Anti-Mad2L1 antibody [EPR9852]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com