Rabbit Polyclonal RGS14 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Regulator of G-protein signaling 14 aa 500 to C-terminus.
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- The Journal of biological chemistry 298:1018362022RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter A Friedman,W Bruce Sneddon,Tatyana Mamonova,Carolina Montanez-Miranda,Suneela Ramineni,Nicholas H Harbin,Katherine E Squires,Julia V Gefter,Clara E Magyar,David R Emlet,John R HeplerPubMed 35307350
- Pathogens (Basel, Switzerland) 8:2019Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelim Çomakli,Selçuk ÖzdemirPubMed 30823555
- Toxicology reports 6:42-502018An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÇiğdem Sevim,Selim Çomaklı,Ali Taghizadehghalehjoughi,Mustafa Özkaraca,Robin Mesnage,Leda Kovatsi,Tatyana I Burykina,Alexandra Kalogeraki,Michael N Antoniou,Aristidis TsatsakisPubMed 30560060
- Toxicology reports 5:125-1332018Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp ( L.).Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelçuk Özdemir,Serdar Altun,Harun ArslanPubMed 29321977
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Regulator of G-protein signaling 14 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43566
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o)-alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance. May be involved in visual memory processing capacity and hippocampal-based learning and memory.
Alternative names
Regulator of G-protein signaling 14, RGS14
Recommended products
Rabbit Polyclonal RGS14 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Regulator of G-protein signaling 14 aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Regulator of G-protein signaling 14 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43566
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RGS14 also known as Regulator of G-protein Signaling 14 serves as a multifunctional scaffold protein that integrates G protein signaling pathways and mitogen-activated protein kinase (MAPK) pathways. It has a molecular weight of approximately 61 kDa. RGS14 is expressed mainly in the brain with high levels in the hippocampus region. It possesses a GoLoco domain two Ras-binding domains and a RGS domain which allows it to interact with various signaling molecules and regulate their activity.
- Biological function summary
RGS14 regulates signal transduction by accelerating the GTPase activity of Gα subunits effectively shortening the duration of G protein signaling. It plays an essential role in modulating synaptic plasticity and memory performance. Researchers have demonstrated RGS14 participates in the regulation of cell signaling by forming complexes with various proteins. This function is particularly significant in the brain where it influences cognition and learning processes.
- Pathways
RGS14 integrates into the MAPK and G-protein pathways affecting downstream signaling cascades. Within the G-protein pathway RGS14 interacts with other proteins like Gαi/o subunits to control signal strength and duration. In the MAPK pathway it links with components such as Ras and Raf modulating the signaling outputs essential for cellular responses. These pathways intersect at several points allowing RGS14 to serve as an important regulatory node.
- Associated diseases and disorders
RGS14 has implications for neurological conditions such as Alzheimer's disease and schizophrenia. Researchers have noted its involvement in cognitive decline linked to Alzheimer's where it might alter interactions with proteins such as amyloid precursor protein (APP). In schizophrenia altered expression or function of RGS14 may affect neurotransmitter signaling in particular glutamatergic pathways involving N-methyl-D-aspartate (NMDA) receptors contributing to the disorder's pathology.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RGS14 antibody (ab96674)
7.5% SDS PAGE
All lanes: Western blot - Anti-RGS14 antibody (ab96674) at 1/500 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 61 kDa
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