AB191500

Anti-RGS20 antibody

Name: Anti-RGS20 antibody (ab191500) | Abcam
Brand: Abcam Limited
SKU: AB191500
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RGS20 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RGS20.

View Alternative Names

RGSZ1, ZGAP1, RGS20, Regulator of G-protein signaling 20, Gz-selective GTPase-activating protein, Regulator of G-protein signaling Z1, Regulator of Gz-selective protein signaling 1, G(z)GAP, Gz-GAP

1 Images

Supplier Data

Western blot - Anti-RGS20 antibody (AB191500)

All lanes:

Western blot - Anti-RGS20 antibody (ab191500) at 1/500 dilution

Lane 1:

HEK293T whole cell lysate

Lane 2:

NIH 3T3 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human RGS20. The exact immunogen used to generate this antibody is proprietary information.

O76081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab191500 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, and the purity is > 95% (by SDS-PAGE).

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RGS20 also known as Regulator of G-protein Signaling 20 is a protein that functions mechanically as a GTPase-activating protein (GAP) for G-alpha subunits of heterotrimeric G proteins. It accelerates the termination of G-protein coupled receptor (GPCR) signaling pathways by increasing the GTPase activity of the G-alpha subunit. The protein has a molecular mass of approximately 26 kDa. RGS20 is expressed in various tissues with high levels in the brain and some expression in peripheral tissues such as the spleen and lung.

Biological function summary

RGS20 modulates signal transduction by accelerating the deactivation of G-proteins which plays an important role in regulating GPCR signaling. It associates with G-beta and G-gamma forming a complex that allows for more efficient signal regulation. The activity of RGS20 is vital in neuronal signaling processes within the brain contributing to regulation of neurotransmitter release and synaptic plasticity.

Pathways

RGS20 influences several G-protein mediated pathways such as the cAMP signaling pathway and the MAPK pathway. It interacts with the G-alpha subunits like G-alpha i and G-alpha q influencing the pathways' duration and intensity. RGS20's regulation of these pathways affects various cellular responses including cell growth and differentiation.

RGS20 relates to neurological disorders and cancer. Altered expression of RGS20 has been observed in some types of brain tumors and neurodegenerative diseases. It is associated with dopamine receptors that are implicated in disorders like schizophrenia and Parkinson's disease. Changes in RGS20 expression or function can affect signaling pathways related to these conditions making it an important protein for research in therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).

See full target information RGS20

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

ACS pharmacology & translational science 6:1817-1828 PubMed38093845

2023

MicroRNA-204-5p Inhibits Hepatocellular Carcinoma by Targeting the Regulator of G Protein Signaling 20.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqing Su,Yao Lu,Honglin An,Jinhong Liu,Feimin Ye,Jiayu Shen,Zhuona Ni,Bin Huang,Jiumao Lin

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 117:109112 PubMed31212130

2019

Regulator of G protein signaling 20 promotes proliferation and migration in bladder cancer via NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Li,Meng Wang,Liangliang Ren,Hanzong Li,Qinghua Liu,Ying Ouyang,Lixin He,Fengyan Li

Oncology reports 39:1948-1956 PubMed29484420

2018

lncRNA NEAT1 promotes cell proliferation and invasion by regulating miR‑365/RGS20 in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Huang,Xin He,Xin-Li Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RGS20 antibody

Western blot - Anti-RGS20 antibody (AB191500)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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