AB131048

Anti-RGS22 antibody [EPR7005]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal RGS22 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications.

View Alternative Names

Regulator of G-protein signaling 22, RGS22

2 Images

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Western blot - Anti-RGS22 antibody [EPR7005] (AB131048)

All lanes:

Western blot - Anti-RGS22 antibody [EPR7005] (ab131048) at 1/1000 dilution

All lanes:

Rat testis lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 147 kDa

Observed band size: 150 kDa

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OI-RD Scanning - Anti-RGS22 antibody [EPR7005] (AB131048)

OI-RD Scanning

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OI-RD Scanning - Anti-RGS22 antibody [EPR7005] (AB131048)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7005

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

This antibody was developed as part of a collaboration with the lab of Damian Romero at the University of Mississippi Medical Center.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RGS22 also known as Regulator of G-Protein Signaling 22 is a member of the RGS protein family with a molecular weight of approximately 155 kDa. This protein modulates the activity of G-proteins by accelerating the deactivation of GTP-bound alpha subunits. RGS22 reduces the lifespan of active G-protein signaling playing a role in signal termination. Expression occurs largely in the testes and it's found in smaller amounts in other tissues like the brain.

Biological function summary

This protein impacts reproductive and neuronal signaling. It forms part of signal transduction complexes that regulate physiological processes. RGS22 interacts with other proteins and cellular components to maintain the proper function of G-protein signaling in specialized tissues. Its activity influences key cellular responses essential for the regulation of several processes particularly in tissues where signaling accuracy requires precise control.

Pathways

RGS22 participates prominently in the G-protein-coupled receptor (GPCR) signaling pathway and the Wnt signaling pathway. Within these pathways RGS22 associates with the G-protein alpha subunits to modulate signal transduction and cellular responses. It interacts with proteins like Gαs and Gαi which are part of the broader network of signaling proteins. These pathways highlight the regulatory role of RGS22 in complex cellular functions and physiological responses.

Abnormal expression or function of RGS22 associates with reproductive disorders and certain neurological diseases. Its dysregulation might contribute to issues like infertility through its impact on signaling in the testes. In neurological contexts RGS22 has been connected to disorders where G-protein signaling balance is disrupted potentially associating with proteins like Gα subunits. Understanding RGS22's role in these diseases could aid in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.

See full target information RGS22

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108022 PubMed37954137

2023

N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Wei,Jingbo Fu,Hailing Zhang,Yan Ling,Xuewu Tang,Shuowu Liu,Miao Yu,Fuyan Liu,Guokun Zhuang,Haihua Qian,Kecheng Zhang,Pinhua Yang,Xinwei Yang,Qi Yang,Shennian Ge,Baohua Zhang,Yexiong Tan,Liang Li,Hongyang Wang

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RGS22 antibody [EPR7005]

Western blot - Anti-RGS22 antibody [EPR7005] (AB131048)

OI-RD Scanning - Anti-RGS22 antibody [EPR7005] (AB131048)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise