AB196799

Anti-RGS5 antibody

Name: Anti-RGS5 antibody (ab196799) | Abcam
Brand: Abcam Limited
SKU: AB196799
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal RGS5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Regulator of G-protein signaling 5.

View Alternative Names

Regulator of G-protein signaling 5, RGS5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody (AB196799)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody (AB196799)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human breast carcinoma tissue sections labeling RGS5 with ab196799 at a 1/50 dilution. Right image treated with sythesized peptide.

Supplier Data

Western blot - Anti-RGS5 antibody (AB196799)

All lanes:

Western blot - Anti-RGS5 antibody (ab196799) at 1/500 dilution

Lane 1:

Extracts from NIH/3T3 cells

Lane 2:

Extracts from NIH/3T3 cells with synthesized peptide

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Regulator of G-protein signaling 5. The exact immunogen used to generate this antibody is proprietary information.

O15539

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Regulator of G-protein Signaling 5 (RGS5) also known as MSTP062 is a 20 kDa protein that modulates G-protein signaling. The protein acts by accelerating the GTPase activity of the alpha subunits of heterotrimeric G-proteins leading to the inactivation of G-protein-coupled receptor (GPCR) signaling. RGS5 shows high expression in vascular smooth muscle cells adipose tissue and the heart. The protein's localization indicates a role in the cardiovascular system and metabolism.

Biological function summary

RGS5 regulates signal transduction pathways by acting as a negative regulator of GPCR signaling and influencing vascular tone and blood pressure. It forms part of the R4 subfamily of RGS proteins recognized for modulating G-protein activity. RGS5 impacts processes like vascular remodeling and angiogenesis by inhibiting G-protein-mediated signal transduction affecting the vascular cell response to proliferation and migration cues.

Pathways

RGS5 plays a role in the GPCR signaling and the PI3K/AKT pathway. The protein interacts closely with G-protein alpha subunits such as Gαq and Gαi during the modulation of these pathways. This interaction helps regulate cardiovascular responses and cellular differentiation events coordinating with other RGS family members to maintain signaling balance.

RGS5 links with hypertension and cancer. In the context of hypertension altered expression of RGS5 correlates with vascular dysfunction affecting blood pressure regulation. In cancer RGS5 influences tumor angiogenesis and growth with potential interactions with proteins such as hypoxia-inducible factor 1-alpha (HIF-1α) affecting the oxygen supply to tumorous tissues. Understanding RGS5's involvement in these conditions can offer targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha (By similarity).

See full target information Regulator of G-protein signaling 5

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:912 PubMed37670124

2023

Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xu,Zhidan Xiang,Weigao E,Yue Lang,Sijia Huang,Weisong Qin,Jingping Yang,Zhaohong Chen,Zhihong Liu

Frontiers in cell and developmental biology 10:927300 PubMed36046337

2022

Single-cell RNA sequencing reveals the cellular and molecular changes that contribute to the progression of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Chen Wang,Zhanjie Fang,Jing Bai,Ying Qian,Yuanyuan Ma,Xiuyan Ruan,Shi Yan,Shaolei Li,Yaqi Wang,Bin Dong,Xin Yang,Meng Li,Xuefeng Xia,Hongzhu Qu,Xiangdong Fang,Nan Wu

BMC endocrine disorders 22:156 PubMed35681135

2022

Implications of regulator of G-protein signaling 5 expression in the pathogenesis of primary and secondary hyperparathyroidism.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Yao Lu,Ling Zhang,Aiping Song,Honglei Zhang,Bo Pang,Jun Liu,Xiaoliang Sun,Haoyang Ji,Linping Huang,Meng Yang

PloS one 16:e0259558 PubMed34748583

2021

NKX2-1 re-expression induces cell death through apoptosis and necrosis in dedifferentiated thyroid carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuko Ito,Fumihiko Furuya,Katsumi Taki,Hideaki Suzuki,Hiroki Shimura

Cell discovery 7:36 PubMed34035226

2021

Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Yiyi Liang,Haiyan Xu,Xiao Zhang,Tiebo Mao,Jiujie Cui,Jiayu Yao,Yongchao Wang,Feng Jiao,Xiuying Xiao,Jiong Hu,Qing Xia,Xiaofei Zhang,Xujun Wang,Yongwei Sun,Deliang Fu,Lei Shen,Xiaojiang Xu,Jing Xue,Liwei Wang

Cancer cell 39:866-882.e11 PubMed33930309

2021

Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulations.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Affo,Ajay Nair,Francesco Brundu,Aashreya Ravichandra,Sonakshi Bhattacharjee,Michitaka Matsuda,LiKang Chin,Aveline Filliol,Wen Wen,Xinhua Song,Aubrianna Decker,Jeremy Worley,Jorge Matias Caviglia,Lexing Yu,Deqi Yin,Yoshinobu Saito,Thomas Savage,Rebecca G Wells,Matthias Mack,Lars Zender,Nicholas Arpaia,Helen E Remotti,Raul Rabadan,Peter Sims,Anne-Laure Leblond,Achim Weber,Marc-Oliver Riener,Brent R Stockwell,Jellert Gaublomme,Josep M Llovet,Raghu Kalluri,George K Michalopoulos,Ekihiro Seki,Daniela Sia,Xin Chen,Andrea Califano,Robert F Schwabe

Cell reports 29:1832-1847.e8 PubMed31722201

2019

Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ross Dobie,John R Wilson-Kanamori,Beth E P Henderson,James R Smith,Kylie P Matchett,Jordan R Portman,Karolina Wallenborg,Simone Picelli,Anna Zagorska,Swetha V Pendem,Thomas E Hudson,Minnie M Wu,Grant R Budas,David G Breckenridge,Ewen M Harrison,Damian J Mole,Stephen J Wigmore,Prakash Ramachandran,Chris P Ponting,Sarah A Teichmann,John C Marioni,Neil C Henderson

Oncology reports 41:165-177 PubMed30365142

2018

RGS5 decreases the proliferation of human ovarian carcinoma‑derived primary endothelial cells through the MAPK/ERK signaling pathway in hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Yan Xu,Lu Feng,Pin Yin,Shuang Shuang Song,Feng Wu,Ping Yan,Zhiqing Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RGS5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody (AB196799)

Western blot - Anti-RGS5 antibody (AB196799)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise