Anti-RGS5 antibody [EPR28539-64] (ab314670)

Rabbit Recombinant Monoclonal RGS5 antibody. Suitable for IHC-P, WB, Dot and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

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Images

Dot Blot - Anti-RGS5 antibody [EPR28539-64] (AB314670), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	Dot	Flow Cyt (Intra)	ICC/IF	IP
Human	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended
Rat	Tested	Not recommended	Expected	Not recommended	Not recommended	Not recommended
Recombinant full length protein - Human	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information RGS5

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha (By similarity).

Alternative names

Regulator of G-protein signaling 5, RGS5

Recommended products

Rabbit Recombinant Monoclonal RGS5 antibody. Suitable for IHC-P, WB, Dot and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28539-64
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for rat WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Regulator of G-protein Signaling 5 (RGS5) also known as MSTP062 is a 20 kDa protein that modulates G-protein signaling. The protein acts by accelerating the GTPase activity of the alpha subunits of heterotrimeric G-proteins leading to the inactivation of G-protein-coupled receptor (GPCR) signaling. RGS5 shows high expression in vascular smooth muscle cells adipose tissue and the heart. The protein's localization indicates a role in the cardiovascular system and metabolism.

Biological function summary

RGS5 regulates signal transduction pathways by acting as a negative regulator of GPCR signaling and influencing vascular tone and blood pressure. It forms part of the R4 subfamily of RGS proteins recognized for modulating G-protein activity. RGS5 impacts processes like vascular remodeling and angiogenesis by inhibiting G-protein-mediated signal transduction affecting the vascular cell response to proliferation and migration cues.

Pathways

RGS5 plays a role in the GPCR signaling and the PI3K/AKT pathway. The protein interacts closely with G-protein alpha subunits such as Gαq and Gαi during the modulation of these pathways. This interaction helps regulate cardiovascular responses and cellular differentiation events coordinating with other RGS family members to maintain signaling balance.

Associated diseases and disorders

RGS5 links with hypertension and cancer. In the context of hypertension altered expression of RGS5 correlates with vascular dysfunction affecting blood pressure regulation. In cancer RGS5 influences tumor angiogenesis and growth with potential interactions with proteins such as hypoxia-inducible factor 1-alpha (HIF-1α) affecting the oxygen supply to tumorous tissues. Understanding RGS5's involvement in these conditions can offer targets for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Dot Blot - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Dot blot analysis of human RGS full length recombinant proteins (lane 1: RGS5, lane 2: RGS4, lane 3: RGS16) using ab314670 at 1:1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human RGS4 and RGS16.
Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The band at approximately 42 kDa may represent a dimer (PMID: 17762159). This has not been confirmed with additional tests.
Exposure time: Lane 1, 2: 125 seconds; Lane 3, 4: 81 seconds
All lanes: Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 4: HL-1 (mouse atrial muscle cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28 kDa, 42 kDa
Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1: 81 seconds; Lane 2-4: 59 seconds
All lanes: Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670) at 1/1000 dilution
Lane 1: Human liver cancer tissue lysate at 20 µg
Lane 2: Human kidney tissue lysate at 20 µg
Lane 3: Human spleen tissue lysate at 20 µg
Lane 4: A-498 (human kidney epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28 kDa, 42 kDa
Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Calu-3(PMID: 25891540). In western blot, anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670) at 1/1000 dilution
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 2: Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 28 kDa
Exposure time: 81s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling RGS5 with ab314670 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive stainig on endothelium and muscularia mucosa of rat colon. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling RGS5 with ab314670 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse kidney. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling RGS5 with ab314670 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive stainig on endothelium and muscularia mucosa of mouse colon. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human breast cancer. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human endometrioid carcinoma. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human kidney. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human spleen. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (ab314670)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive stainig on endothelium and muscularis mucosa of human colon. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.