Anti-RGS5 antibody [EPR28539-64]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast cancer. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive stainig on endothelium and muscularis mucosa of human colon. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human endometrioid carcinoma. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling RGS5 with ab314670 at 1/500 (0.992 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling RGS5 with ab314670 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling RGS5 with ab314670 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive stainig on endothelium and muscularia mucosa of mouse colon. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling RGS5 with ab314670 at 1/2000 (0.248 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive stainig on endothelium and muscularia mucosa of rat colon. The section was incubated with ab314670 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

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Western blot - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Calu-3(PMID : 25891540). In western blot, anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa

false

Exposure time: 81s

• WB

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Western blot - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1 : 81 seconds; Lane 2-4 : 59 seconds

All lanes:

Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670) at 1/1000 dilution

Lane 1:

Human liver cancer tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

A-498 (human kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa,42 kDa

false

• Dot

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Dot Blot - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Dot blot analysis of human RGS full length recombinant proteins (lane 1 : RGS5, lane 2 : RGS4, lane 3 : RGS16) using ab314670 at 1 : 1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody does not cross-react with human RGS4 and RGS16.

• WB

Supplier Data

Western blot - Anti-RGS5 antibody [EPR28539-64] (AB314670)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The band at approximately 42 kDa may represent a dimer (PMID : 17762159). This has not been confirmed with additional tests. Exposure time : Lane 1, 2 : 125 seconds; Lane 3, 4 : 81 seconds

All lanes:

Western blot - Anti-RGS5 antibody [EPR28539-64] (ab314670) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 4:

HL-1 (mouse atrial muscle cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa,42 kDa

false