AB128943

Anti-RGS6 antibody [EPR6342]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RGS6 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

Regulator of G-protein signaling 6, RGS6, S914

3 Images

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Western blot - Anti-RGS6 antibody [EPR6342] (AB128943)

All lanes:

Western blot - Anti-RGS6 antibody [EPR6342] (ab128943) at 1/1000 dilution

Lane 1:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

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Lab

Western blot - Anti-RGS6 antibody [EPR6342] (AB128943)

All lanes:

Western blot - Anti-RGS6 antibody [EPR6342] (ab128943) at 1/1000 dilution

Lane 1:

mouse brain

Lane 2:

rat brain

Predicted band size: 54 kDa

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Exposure time: 3min

OI-RD Scanning - Anti-RGS6 antibody [EPR6342] (AB128943)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-RGS6 antibody [EPR6342] (AB128943)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-RGS6 antibody [EPR6342] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6342

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Regulators of G-protein Signaling 6 (RGS6) is a multifunctional protein involved in the negative regulation of G-protein-coupled receptor signaling pathways. Commonly known as RGS6 this protein exhibits a mass of approximately 52 kDa. It is widely expressed in many tissues including the brain heart and liver. RGS6 contains a common RGS domain that accelerates the GTPase activity of G-alpha subunits facilitating the return of these subunits to their inactive GDP-bound form.

Biological function summary

RGS6 contributes to the fine-tuning of cellular signaling processes. It plays a significant role in nervous system function by modulating neurotransmitter release and signal transduction. RGS6 is part of a signaling complex that includes G-protein beta and gamma subunits which further refines its regulatory activity. This complex interaction helps to ensure precise control over timing and intensity of signal propagation in cells.

Pathways

RGS6 is an important component of the G-protein signaling pathway interacting with G-alpha subunits. It also has involvement in the MAPK/ERK signaling pathway where it influences cellular responses such as proliferation and differentiation. Related proteins in these pathways include other RGS family members and various MAP kinases that collaborate to maintain cellular homeostasis and response to environmental stimuli.

RGS6 has implications in conditions such as depression and cancer. It modulates pathways linked to these disorders for instance mood regulation and tumor proliferation. Through these functions RGS6 interacts with proteins like G-protein-coupled receptors and specific transcription factors associated with these diseases offering potential therapeutic targets for treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity (PubMed : 10521509).

See full target information RGS6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 7:e009179 PubMed30371330

2018

Regulator of G Protein Signaling 6 Facilities Cardiac Hypertrophy by Activating Apoptosis Signal-Regulating Kinase 1-P38/c-JUN N-Terminal Kinase 1/2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Huang,Jingxian Shu,Weihong Jiang,Mengqing Jiang,Yao Lu,Haijiang Dai,Nana Xu,Hong Yuan,Jingjing Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RGS6 antibody [EPR6342]

Western blot - Anti-RGS6 antibody [EPR6342] (AB128943)

Western blot - Anti-RGS6 antibody [EPR6342] (AB128943)

OI-RD Scanning - Anti-RGS6 antibody [EPR6342] (AB128943)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise