Rabbit Polyclonal RGS7 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RGS7.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RGS7. The exact immunogen used to generate this antibody is proprietary information. Database link P49802
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Target data
Function
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:10521509, PubMed:10862767). The RGS7/GNB5 dimer enhances GNAO1 GTPase activity (PubMed:10521509). May play a role in synaptic vesicle exocytosis (PubMed:12659861). Modulates the activity of potassium channels that are activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling (PubMed:15897264).
Alternative names
Regulator of G-protein signaling 7, RGS7, RGS7
Recommended products
Rabbit Polyclonal RGS7 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RGS7.
Alternative names
Regulator of G-protein signaling 7, RGS7, RGS7
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RGS7. The exact immunogen used to generate this antibody is proprietary information. Database link P49802
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Regulator of G protein signaling 7 (RGS7) also known as RGP7 is a member of the R7 family of RGS proteins with a molecular weight of approximately 85 kDa. RGS7 regulates G protein signaling by accelerating the deactivation of Gα subunits through its GTPase-activating protein activity. It is widely expressed in the central nervous system especially in the brain regions like the hippocampus and basal ganglia.
- Biological function summary
G protein-coupled receptor (GPCR) signaling modulation is an important role of RGS7. It forms a complex with the Gβ5 subunit and other anchoring proteins to regulate synaptic transmission and neuronal excitability. This complex localizes to synaptic sites and modifies neurotransmitter release which can influence cognitive functions and behaviors. RGS7 helps in maintaining signal fidelity within neural circuits.
- Pathways
G protein-coupled receptor signaling and neurotransmitter release are two important biological pathways involving RGS7. In the GPCR signaling pathway RGS7 modulates GPCR response by interacting with Gα subunits to accelerate their inactivation. Interaction with neurotransmitter release pathways occurs as RGS7 controls calcium influx and synaptic vesicle fusion. It relates to proteins such as Gβ5 and other G protein subunits playing key roles in these pathways.
- Associated diseases and disorders
Research indicates that RGS7 is connected to certain neurological conditions such as addiction and depression. Dysregulation of RGS7 may alter neurotransmitter systems influencing susceptibility to addictive substances and mood disorders. RGS7 interacts with proteins such as Gβ5 which are also involved in the dysregulation seen in these conditions. Understanding the functions of RGS7 can inform therapeutic strategies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-RGS7 antibody (ab228618)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-RGS7 antibody (ab228618) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 58 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RGS7 antibody (ab228618)
Ice-cold methanol-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for RGS7 (green) using ab228618 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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