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AB108975

Anti-RGS9 antibody [EPR2873]

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(1 Publication)

Rabbit Recombinant Monoclonal RGS9 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Regulator of G-protein signaling 9, RGS9

6 Images
Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (AB108975)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (AB108975)

Intracellular flow cytometric analysis ofpermeabilized PC12 cells labelling RGS9with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (AB108975)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] (AB108975)

Intracellular Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluorr® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] (AB108975)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] (AB108975)

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291 a mouse anti-tubulin (1/1000) and ab150120 an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody ab150120 an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody ab150077 an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

Western blot - Anti-RGS9 antibody [EPR2873] (AB108975)
  • WB

Unknown

Western blot - Anti-RGS9 antibody [EPR2873] (AB108975)

Note : Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution

Lane 1:

PC12 lysates at 10 µg

Lane 2:

Human spleen lysates at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

Western blot - Anti-RGS9 antibody [EPR2873] (AB108975)
  • WB

Lab

Western blot - Anti-RGS9 antibody [EPR2873] (AB108975)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution

Lane 1:

Y79 whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

Western blot - Anti-RGS9 antibody [EPR2873] (AB108975)
  • WB

Lab

Western blot - Anti-RGS9 antibody [EPR2873] (AB108975)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (ab108975) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

  • Carrier free

    Anti-RGS9 antibody [EPR2873] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2873

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30 - 1/60", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30 - 1/60", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Regulator of G protein Signaling 9 or RGS9 plays an important role in the regulation of G protein-coupled receptor (GPCR) signaling pathways. RGS9 acts as a GTPase-activating protein (GAP) accelerating the deactivation of Gα subunits which helps terminate the signal transduction. This protein with a molecular mass of roughly 73 kDa is highly expressed in the brain especially in the striatum and in the retina. These tissues suggest its specialized involvement in visual and neurological functions.
Biological function summary

RGS9 functions as an important regulator within multiple signaling complexes. It associates with RGS9-binding protein (R9AP) and Gβ5 subunits to form larger complexes that enhance its activity. RGS9 regulates phototransduction in rod and cone photoreceptors by controlling the inactivation of transducin a heterotrimeric G protein involved in visual processing. This regulation ensures proper recovery of photoreceptors after light-induced activation maintaining visual acuity and stability.

Pathways

RGS9 is integral to visual signal transduction and dopaminergic signaling pathways. In the visual system it modulates the phototransduction cascade by interacting with transducin within the rod outer segment. Within dopamine pathways it affects signaling by modulating receptors like D2 dopamine receptors involving proteins such as Gαolf which mediates cAMP production. These interactions regulate neuronal responses to various stimuli impacting vision and limbic system functions.

RGS9 abnormalities relate to conditions like bradyopsia and drug addiction. Bradyopsia characterized by slow vision recovery after light exposure is linked to RGS9 mutations which disrupt normal photoreceptor recovery. In addiction altered RGS9 expression impacts dopamine signaling affecting addiction pathways with involvement of D2 dopamine receptors. Together these insights highlight RGS9's critical role in maintaining normal visual and neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to GNAT1. Involved in phototransduction; key element in the recovery phase of visual transduction (By similarity).
See full target information Regulator of G-protein signaling 9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 12:1395-406 PubMed23268931

2012

Isotope coded protein labeling coupled immunoprecipitation (ICPL-IP): a novel approach for quantitative protein complex analysis from native tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Vogt,Bettina Fuerholzner,Norbert Kinkl,Karsten Boldt,Marius Ueffing
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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