Anti-RGS9 antibody [EPR2873] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Y79 cells labelling RGS9 with purified ab108975 at a dilution of 1/60 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling RGS9 with purified ab108975 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized PC12 cells labelling RGS9 with unpurified ab108975 (red) at a dilution of 1/30 or a rabbit IgG (negative) (green).

• WB

Unknown

Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Note : Predicted molecular weights for UniProt ID O75916 isoforms 1 to 4 are ~77, 55, 57 and 50 kDa respectively. Therefore, the observed WB band may correspond to isoform 2, 3 or 4.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (<a href='/en-us/products/primary-antibodies/rgs9-antibody-epr2873-ab108975'>ab108975</a>) at 1/1000 dilution

Lane 1:

PC12 lysates at 10 µg

Lane 2:

Human spleen lysates at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

• WB

Lab

Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (<a href='/en-us/products/primary-antibodies/rgs9-antibody-epr2873-ab108975'>ab108975</a>) at 1/1000 dilution

Lane 1:

Y79 whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false

• WB

Lab

Western blot - Anti-RGS9 antibody [EPR2873] - BSA and Azide free (AB247728)

This data was developed using ab108975, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RGS9 antibody [EPR2873] (<a href='/en-us/products/primary-antibodies/rgs9-antibody-epr2873-ab108975'>ab108975</a>) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 55 kDa

false