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AB249187

Anti-RHAG antibody [EPR10011] - BSA and Azide free

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Rabbit Recombinant Monoclonal RHAG antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CD241, RH50, RHAG, Ammonium transporter Rh type A, Erythrocyte membrane glycoprotein Rh50, Erythrocyte plasma membrane 50 kDa glycoprotein, Rhesus blood group family type A glycoprotein, Rhesus blood group-associated ammonia channel, Rhesus blood group-associated glycoprotein, Rh50A, Rh family type A glycoprotein, Rh type A glycoprotein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)

This data was developed using ab155094, the same antibody clone in a different buffer formulation.

ab155094 showing +ve staining in Human normal colon.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)

This data was developed using ab155094, the same antibody clone in a different buffer formulation.

ab155094 showing +ve staining in Human normal heart.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)

This data was developed using ab155094, the same antibody clone in a different buffer formulation.

ab155094 showing +ve staining in Human normal tonsil.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)
  • WB

Unknown

Western blot - Anti-RHAG antibody [EPR10011] - BSA and Azide free (AB249187)

This data was developed using ab155094, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RHAG antibody [EPR10011] (<a href='/en-us/products/primary-antibodies/rhag-antibody-epr10011-ab155094'>ab155094</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney cell lysate at 10 µg

Lane 2:

Human fetal liver cell lysate at 10 µg

Lane 3:

Human bone marrow cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10011

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab249187 is the carrier-free version of ab155094.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RHAG protein also known as the Rh-associated glycoprotein or Rh family C glycoprotein has a molecular mass of approximately 50000 Daltons. It is present mainly on erythrocyte membranes and works as a part of the Rh blood group system. RHAG acts as an ammonium transporter enabling the movement of ammonia and possibly CO2 across the cell membrane though exact specifics of its mechanisms are not completely clear.
Biological function summary

The RHAG protein is part of the Rh complex within the erythrocyte membrane which also includes RhD and RhCE proteins. This complex contributes to the structural integrity of the red cell membrane and plays an important role in maintaining the biconcave shape of the erythrocytes which is necessary for optimal oxygen exchange and gas transport. RHAG's function is essential for normal erythrocyte operation and overall cellular homeostasis.

Pathways

RHAG participates in the ammonia transport pathway and is linked to the Rh protein complex pathway. It interacts closely with proteins like RhD and RhCE working together to facilitate membrane stability and function in red blood cells. These interactions within the pathways emphasize RHAG's role in gas transport-related functions and metabolic waste management in the bloodstream.

Defects in RHAG expression or function lead to conditions like Rh-null syndrome and hereditary stomatocytosis. This syndrome characterized by a lack of all Rh antigens on red blood cells often associates with hemolytic anemia due to the compromised integrity of the erythrocyte membrane. Mutations in RHAG also relate to interactions with other proteins like SLC4A1 another important protein involved in red blood cell homeostasis enhancing the complexity and clinical significance of the RHAG protein in health and disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed : 35835865). Heterotrimer with RHCE (RHAG)2(RHCE), that transports ammonium and its related derivative methylammonium, in both neutral and ionic forms, across the erythrocyte membrane (PubMed : 11062476, PubMed : 11861637, PubMed : 15572441, PubMed : 15856280, PubMed : 19273840, PubMed : 21849667, PubMed : 22012326, PubMed : 24077989, PubMed : 26354748). The transport of NH4(+) is electrogenic and masks the NH3 transport (PubMed : 26354748). Also, may act as a CO2 channel (PubMed : 17712059, PubMed : 19273840, PubMed : 24077989). In vitro, leaks monovalent cations (PubMed : 18931342, PubMed : 21849667). Moreover in erythrocyte, regulates RHD membrane expression (PubMed : 12130520) and is associated with rhesus blood group antigen expression (PubMed : 12130520, PubMed : 19744193).
See full target information RHAG
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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