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AB220043

Anti-RHBG antibody

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(1 Publication)

Rabbit Polyclonal RHBG antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RHBG aa 350-400.

View Alternative Names

Ammonium transporter Rh type B, Rhesus blood group family type B glycoprotein, Rh family type B glycoprotein, Rh type B glycoprotein, RHBG

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RHBG antibody (AB220043)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RHBG antibody (AB220043)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized HepG2 cells labeling RHBG with ab220043 at 4 μg/ml (green).

Western blot - Anti-RHBG antibody (AB220043)
  • WB

Supplier Data

Western blot - Anti-RHBG antibody (AB220043)

All lanes:

Western blot - Anti-RHBG antibody (ab220043) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RHBG aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q9H310

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHBG also known as Rh family B glycoprotein is an ammonia transporter. This protein moves ammonia across cell membranes without the use of energy facilitating its passage in and out of cells. RHBG weighs approximately 53 kDa and is integral to membrane structure. You can find RHBG highly expressed in the kidney and liver where it performs its ammonia transport function efficiently. It also shows expression in parts of the gastrointestinal tract contributing to nitrogen metabolism.
Biological function summary

RHBG functions as part of processes that regulate acid-base balance in the body maintaining homeostasis. Although not part of a larger complex RHBG works alongside other ammonia transporters such as its close relatives RHCG and RHAG to manage ammonium balance in tissues. This action is critical in waste excretion and nitrogen metabolism allowing proper cell function.

Pathways

Mechanisms involving RHBG play roles in the nitrogen metabolism and renal acid excretion pathways. In the kidney it operates closely with pathways that balance pH levels meaning it cooperates with proteins like RHCG to achieve effective regulation of ammonia levels within the renal system. These pathways support the safe removal of waste products from the body protecting against metabolic disorders caused by nitrogen imbalance.

RHBG associates with conditions like hyperammonemia and certain kidney diseases. When RHBG function is impaired ammonia can accumulate leading to elevated blood ammonia levels contributing to various pathologies. In the context of kidney diseases RHBG disruption interferes with the body's ability to maintain proper acid-base homeostasis linking it to proteins such as RHCG and others involved in bicarbonate transport that help regulate kidney function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the basolateral plasma membrane of epithelial cells likely contributing to renal transepithelial ammonia transport and ammonia metabolism. May transport either NH4(+) or NH3 ammonia species predominantly mediating an electrogenic NH4(+) transport (PubMed : 15284342, PubMed : 15929723, PubMed : 24077989). May act as a CO2 channel providing for renal acid secretion (PubMed : 24077989).
See full target information RHBG
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Publications (1)

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Molecular medicine (Cambridge, Mass.) 31:78 PubMed40000966

2025

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Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Ding,Hua-Ying Zhang,Jia-Hao Chen,Meng-Jie Yang,Zhi-Xiong Huang,Yi-Hong Lei,Qin-Kun Sun,Jian-Bin Bai,Dian-Chao Lin,Jian-Fa Lan,Lu-Lu Ren,Zheng-Yi Chen,Wei-Dong Zhou,Qiong-Hua Chen
View all publications
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Product promise

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