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AB281911

Anti-RhD antibody [D7C2] - Rabbit IgG (Chimeric)

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Rabbit Recombinant Monoclonal RHD antibody. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

CD240D, Blood group Rh(D) polypeptide, RHXIII, Rh polypeptide 2, Rhesus D antigen, RhPII, RHD

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] - Rabbit IgG (Chimeric) (AB281911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] - Rabbit IgG (Chimeric) (AB281911)

Immunofluorescence analysis of paraformaldehyde fixed K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling RhD with ab281911 at 10 μg/ml for 1hour, followed by Alexa Fluor® 488 secondary antibody at 2 μg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. K562 cells were immobilized on Shi-fix™ coverslips.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

D7C2

Isotype

IgG

Light chain type

lambda

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab281911 is the chimeric rabbit version of ab281912.

This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RhD protein also known as the Rhesus D antigen is a transmembrane protein that functions as a transporter across erythrocyte membranes. The protein has a mass of approximately 30 kDa. It is a part of the Rh blood group system and is most prominently expressed on the surface of red blood cells. The presence or absence of the RhD antigen on red blood cells defines the Rh positive or negative blood type which is important in transfusion medicine and pregnancy.
Biological function summary

The RhD protein is involved in transporting ammonium and carbon dioxide across the erythrocyte membrane. Although it is part of a larger Rh protein complex which consists of other Rh-associated glycoproteins RhD itself plays a critical role in maintaining the structural integrity and physiological function of red blood cells. The expression levels of RhD can affect the immunogenic response in situations like pregnancy where maternal and fetal blood types may differ.

Pathways

The RhD protein interacts with the RhCE protein to form complexes that participate in the Rh blood group system. This system is integral to the red blood cell membrane's structural organization and is linked to cellular processes that influence erythrocyte shape and deformability. The RhD protein also has interactions along pathways related to the anion exchanger protein AE1 impacting red cell ion transport and gas exchange.

Several conditions link to RhD notably hemolytic disease of the newborn and autoimmune hemolytic anemia. Hemolytic disease of the newborn occurs when an Rh-negative mother carries an Rh-positive fetus leading to maternal sensitization to the RhD antigen. This can result in maternal antibodies crossing the placenta and attacking fetal red blood cells. Autoimmune hemolytic anemia may also involve RhD if RhD-targeted antibodies are produced leading to destruction of red blood cells similar to the effect seen with other membrane proteins like the glycophorin C.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
See full target information RHD
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Product promise

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For full details, please see our Terms & Conditions

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