Anti-Rho antibody [EP487Y]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [EP487Y] (AB40673)

Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab40673 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rho antibody [EP487Y] (AB40673)

Immunofluorescence staining of MCF7 cells with purified ab40673 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used as primary, followed by an Alexa Fluor® 488 goat anti-rabbit secondary (ab150077) at a dilution of 1/500.

• WB

Lab

Western blot - Anti-Rho antibody [EP487Y] (AB40673)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

All lanes:

MDA-MB-435 at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L)

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

• WB

Lab

Western blot - Anti-Rho antibody [EP487Y] (AB40673)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Lane 5:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

• WB

Lab

Western blot - Anti-Rho antibody [EP487Y] (AB40673)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/5000 dilution

All lanes:

HL-60 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

• WB

Lab

Western blot - Anti-Rho antibody [EP487Y] (AB40673)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

All lanes:

C6 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

• WB

Lab

Western blot - Anti-Rho antibody [EP487Y] (AB40673)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

All lanes:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

• WB

CiteAb

Western blot - Anti-Rho antibody [EP487Y] (AB40673)

Rho western blot using anti-Rho antibody [EP487Y] ab40673. Publication image and figure legend from Wang, Z., Li, T. E., et al., 2020, Aging (Albany NY), PubMed 31986487.

ab40673 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab40673 please see the product overview.

The Rho/ROCK1 pathway participated in CNN1-induced breast cancer. (A) The half-time of ROCK1 transcript after CNN1 overexpression was decreased using half-time assay. The MCF-7 and MDA-MB-231 cells after transfection with CNN1 overexpression were treated with Act D (8 μg/ml) for 0 h, 2 h, 4 h, 6 h, and 8 h. The ROCK1 mRNA remaining was detected by qRT-PCR. (B) The protein expressions of Rho and ROCK1 was decreased after CNN1 overexpression. The western blot assay was used to measure the protein expression after CNN1 overexpression for 24 h. Control, the cells were cultured without any treatment. NC, the cells were treated with the negative control. CNN1 OE, the cells were treated with CNN1 overexpression. *p<0.05 vs. Control and **p<0.001 vs. Control.

false