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Rabbit Recombinant Monoclonal RHOA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Rho antibody [EP487Y] (AB40673), expandable thumbnail
  • Western blot - Anti-Rho antibody [EP487Y] (AB40673), expandable thumbnail
  • Western blot - Anti-Rho antibody [EP487Y] (AB40673), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [EP487Y] (AB40673), expandable thumbnail
  • Western blot - Anti-Rho antibody [EP487Y] (AB40673), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

-

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle (PubMed:23871831). Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers (PubMed:31570889, PubMed:8910519, PubMed:9121475). Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis (PubMed:12900402, PubMed:16236794). Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion (PubMed:20974804, PubMed:23940119). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (PubMed:19934221). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:19403695, PubMed:9635436). Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center (PubMed:31888991). May be an activator of PLCE1 (PubMed:16103226). In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity). When activated by DAAM1 may signal centrosome maturation and chromosomal segregation during cell division. May also be involved in contractile ring formation during cytokinesis.(Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague.

Alternative names

ARH12, ARHA, RHO12, RHO12, RHOA, ARH12, ARHA, Transforming protein RhoA, Rho cDNA clone 12, h12

Recommended products

Rabbit Recombinant Monoclonal RHOA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP487Y

Purification technique

Affinity purification Protein A

Specificity

This antibody is specific for Rho. It is also expected to detect RhoA, RhoB and RhoC.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rho is a small GTPase known for its role in regulating the cytoskeleton. Alternate names include RhoA RhoB and RhoC. Rho proteins have a molecular mass of about 21 kDa. It is expressed in various tissues with particularly high levels in muscle the brain and fibroblasts. Rho belongs to the Ras homologous (Rho) family of GTPases and acts as a molecular switch that toggles between an active GTP-bound state and an inactive GDP-bound state.

Biological function summary

By controlling the organization of the cytoskeleton Rho plays a vital role in cell shape motility and division. It is part of the Rho family GTPase complex which includes other members like Rac and Cdc42. These proteins coordinate actin filament formation affecting cellular movements such as migration and adhesion. Rho also influences the formation of stress fibers and focal adhesions linking it to mechanical stress response and cell polarity.

Pathways

Rho orchestrates cell activities through pathways like the Rho/Rho kinase (ROCK) and the phosphatidylinositol-45-bisphosphate 3-kinase (PI3K) pathways. In the Rho/ROCK pathway Rho activates ROCK leading to actomyosin contractility and cell movement. Rho also interacts with proteins like Rac and Cdc42 in the PI3K pathway coordinating diverse cellular responses. This involvement is important for processes such as cell cycle progression and apoptosis.

Associated diseases and disorders

Improper Rho functioning is linked to conditions like cancer and cardiovascular diseases. Rho proteins contribute to cancer progression through their role in regulating cell motility and invasion with an overexpression often seen in tumors. Additionally in cardiovascular diseases Rho affects vascular smooth muscle function influencing blood pressure regulation. In these contexts Rho interacts with proteins such as ROCK and Rac which are also implicated in pathological states. Researchers use anti-Rho and anti-Rho antibodies to study these interactions and understand Rho-related pathologies better.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rho antibody [EP487Y] (ab40673)

    Immunofluorescence staining of MCF7 cells with purified ab40673 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used as primary, followed by an Alexa Fluor® 488 goat anti-rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/500.

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Western blot - Anti-Rho antibody [EP487Y] (ab40673)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

    Lane 1: A431 cell lysate at 20 µg

    Lane 2: MCF7 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: K562 cell lysate at 20 µg

    Lane 5: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Western blot - Anti-Rho antibody [EP487Y] (ab40673)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

    All lanes: Mouse kidney tissue lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [EP487Y] (ab40673)

    Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab40673 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Western blot - Anti-Rho antibody [EP487Y] (ab40673)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

    All lanes: C6 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Western blot - Anti-Rho antibody [EP487Y] (ab40673)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/5000 dilution

    All lanes: HL-60 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673), expandable thumbnail

    Western blot - Anti-Rho antibody [EP487Y] (ab40673)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution

    All lanes: MDA-MB-435 at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L)

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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