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AB154785

Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950]

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(2 Publications)

Rabbit Recombinant Monoclonal Rho guanine exchange factor 16/NBR antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

EPHEXIN4, NBR, ARHGEF16, Rho guanine nucleotide exchange factor 16, Ephexin-4

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • WB

Unknown

Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

All lanes:

Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 80 kDa

false

Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)
  • WB

Unknown

Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (AB154785)

Blocking and Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution

All lanes:

Mouse kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9950

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rho guanine exchange factor 16 also known as ARHGEF16 or NBR is a protein that acts as an exchange factor for Rho GTPases by facilitating the exchange of GDP for GTP. This protein has a molecular mass of approximately 100 kDa. It is widely expressed in various tissues with higher levels found in the brain and lungs. The alternative names for this protein "guanine exchange factor" and "anti-ARHGEF16" reflect its involvement in activating small GTPase by swapping GDP with GTP influencing several cellular processes.
Biological function summary

Rho guanine exchange factor 16 plays a role in signal transduction pathways that control cytoskeleton dynamics cell migration and growth. It often functions as part of a larger protein complex involved in cellular response to external stimuli. By interacting with and activating Rho family GTPases like RhoA it contributes to the reorganization of actin filaments and the regulation of cell shape and motility which are critical for tissue remodeling and development.

Pathways

Rho guanine exchange factor 16 participates in pathways such as Rho GTPase cycle and endothelial barrier maintenance. It is closely associated with RhoA within these pathways affecting cell cycle regulation and cellular stress responses. This protein ensures the activation of downstream effectors facilitating changes required for cellular adherence and survival under various physiological conditions.

Dysregulation or mutation of Rho guanine exchange factor 16 links to cancer progression and neurological disorders. Abnormal signaling through this target can lead to altered actin dynamics affecting cell division and migration seen in tumor metastasis. Associated proteins such as RhoA also play roles in these diseases highlighting the complex interaction networks involving Rho guanine exchange factor 16 that can influence disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
See full target information ARHGEF16
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 12:3443-3452 PubMed38192976

2024

ARHGEF16 expression correlates with proliferation, migration and invasion of colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Liu,Dong Xu,Baocheng Zhu,Chao Song,Qianzhi Ni,Zhenjun Gao

Molecular biology of the cell 34:ar124 PubMed37703099

2023

EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events.

Applications

Unspecified application

Species

Unspecified reactive species

Mathis Neuhaus,Claes Fryklund,Holly Taylor,Andrea Borreguero-Muñoz,Franziska Kopietz,Hamidreza Ardalani,Oksana Rogova,Laura Stirrat,Shaun K Bremner,Peter Spégel,Nia J Bryant,Gwyn W Gould,Karin G Stenkula
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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