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Rabbit Recombinant Monoclonal RHOA antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 95 publications.


Images

Western blot - Anti-RhoA antibody [EPR18134] (AB187027), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [EPR18134] (AB187027), expandable thumbnail
  • Western blot - Anti-RhoA antibody [EPR18134] (AB187027), expandable thumbnail
  • Western blot - Anti-RhoA antibody [EPR18134] (AB187027), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

1 product for Alternative Version

Target data

Function

Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle (PubMed:23871831). Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers (PubMed:31570889, PubMed:8910519, PubMed:9121475). Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis (PubMed:12900402, PubMed:16236794). Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion (PubMed:20974804, PubMed:23940119). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (PubMed:19934221). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:19403695, PubMed:9635436). Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center (PubMed:31888991). May be an activator of PLCE1 (PubMed:16103226). In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity). When activated by DAAM1 may signal centrosome maturation and chromosomal segregation during cell division. May also be involved in contractile ring formation during cytokinesis. (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague.

Alternative names

ARH12, ARHA, RHO12, RHOA, Transforming protein RhoA, Rho cDNA clone 12, h12

Recommended products

Rabbit Recombinant Monoclonal RHOA antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 95 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18134
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RhoA also known as Ras homolog family member A is a small signaling GTPase. It weighs approximately 21 kDa. This protein is a part of the Rho family which includes other members like RhoB and RhoC. RhoA is widely expressed in various tissues including the brain kidney liver and lungs. Mechanically RhoA functions as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. This activity regulates the organization of the actin cytoskeleton and influences cell shape attachment and motility.

Biological function summary

The role of RhoA extends to cell proliferation and differentiation. It is a component of the Rho-GTPase cycle interacting with numerous effectors to transmit signals from the cell membrane to the actin cytoskeleton. RhoA influences the assembly of stress fibers and focal adhesions. It plays a part in cellular adhesion and migration making it essential for development and wound healing processes. As a central player in these activities RhoA affects several cellular responses to external stimuli.

Pathways

Research places RhoA prominently in the regulation of the Rho-ROCK (Rho-associated protein kinase) pathway and the planar cell polarity pathway. The Rho-ROCK pathway contributes to cytoskeletal dynamics and cell contractility while the planar cell polarity pathway influences tissue architecture during embryonic development. RhoA interacts with G17A in these pathways coordinating activities such as smooth muscle contraction and neuronal growth. These interactions also involve cross-talk with other signals linked to GTPases like Rac1 and Cdc42.

Associated diseases and disorders

RhoA has connections to cancer progression and hypertension. In cancer RhoA regulates actin dynamics influencing tumor cell migration and invasion. It forms interactions with proteins such as p53 affecting apoptotic pathways and tumor suppression. In hypertension RhoA's role in smooth muscle contraction affects blood vessel tension and blood pressure regulation. Abnormal activity in the Rho-ROCK pathway contributes to these disorders highlighting the significance of RhoA in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Western blot - Anti-RhoA antibody [EPR18134] (ab187027)

    Lanes 1- 4: Merged signal (red and green). Green - ab187027 observed at 21 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.

    ab187027 was shown to react with RhoA in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human RHOA knockout HEK-293T cell line ab266592 (knockout cell lysate Human RHOA knockout HEK-293T cell lysate ab257637) was used. Wild-type HEK-293T and RHOA knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187027 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-RhoA antibody [EPR18134] (ab187027) at 1/5000 dilution

    Lanes 1 and 3: Wild-type HEK-293T cell lysate at 20 µg

    Lanes 2 and 4: RHOA knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human RHOA knockout HEK-293T cell line (Human RHOA knockout HEK-293T cell line ab266592)

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [EPR18134] (ab187027)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RhoA with ab187027 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab187027 at 1/150 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Western blot - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Western blot - Anti-RhoA antibody [EPR18134] (ab187027)

    5% NFDM/TBST: Blocking and diluting buffer.

    Human RhoA full length protein (ab101594) contains aa1-193 with His-Tag® at N-Terminus; Human RhoB full length protein (Recombinant Human RHOB protein ab107139) contains aa1-193 with His-Tag® at N-Terminus; Human RhoC full length protein (Recombinant Human RHOC protein ab98085) contains aa1-190 with His-Tag® at N-Terminus.

    All lanes: Western blot - Anti-RhoA antibody [EPR18134] (ab187027) at 1/5000 dilution

    Lane 1: Human RhoA full length protein at 0.01 µg

    Lane 2: Human RhoB full length protein at 0.01 µg

    Lane 3: Human RhoC full length protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

    Exposure time: 1s

  • Western blot - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Western blot - Anti-RhoA antibody [EPR18134] (ab187027)

    Lanes 1 - 3: Merged signal (red and green). Green - ab187027 observed at 22 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab187027 was shown to specifically react with RhoA in wild-type Hek293T cells as signal was lost in RHOA knockout cells. Wild-type and RHOA knockout samples were subjected to SDS-PAGE. ab187027 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-RhoA antibody [EPR18134] (ab187027) at 1/1000 dilution

    Lane 1: Wild-type Hek293T whole cell lysate at 20 µg

    Lane 2: RHOA knockout Hek293T whole cell lysate at 20 µg

    Lane 3: Jurkat whole cell lysate at 20 µg

    Predicted band size: 22 kDa

  • Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (ab187027)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling RhoA with ab187027 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and a unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Western blot - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Western blot - Anti-RhoA antibody [EPR18134] (ab187027)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-RhoA antibody [EPR18134] (ab187027) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

    Exposure time: 3min

  • Western blot - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Western blot - Anti-RhoA antibody [EPR18134] (ab187027)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-RhoA antibody [EPR18134] (ab187027) at 1/5000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

    Exposure time: 3min

  • Western blot - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Western blot - Anti-RhoA antibody [EPR18134] (ab187027)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-RhoA antibody [EPR18134] (ab187027) at 1/5000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Rat brain lysate at 10 µg

    Lane 5: Rat kidney lysate at 10 µg

    Lane 6: Rat spleen lysate at 10 µg

    Lane 7: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 8: Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 9: NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [EPR18134] (ab187027), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [EPR18134] (ab187027)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling RhoA with ab187027 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab187027 at 1/150 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

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