Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] - BSA and Azide free (AB324243)

This data was developed using ab324028, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 27043630).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Rho A + B + C antibody [EPR18299] staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] (<a href='/en-us/products/primary-antibodies/rhoa-rhob-adp-ribosylated-antibody-vif140-a1-ab324028'>ab324028</a>) at 1/1000 dilution

Lane 1:

Untreated HT-22 (mouse hippocampal neuronal cell) whole cell lysate at 20 µg

Lane 2:

HT-22 treated with 500nM C3 for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Observed band size: 22 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] - BSA and Azide free (AB324243)

This data was developed using ab324028, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 27043630).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-Rho A + B + C antibody [EPR18299] staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] (<a href='/en-us/products/primary-antibodies/rhoa-rhob-adp-ribosylated-antibody-vif140-a1-ab324028'>ab324028</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 2:

293T transfected with an empty vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Lane 3:

293T transfected with an human RhoA expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 4:

293T transfected with an human RhoA expression vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Lane 5:

293T transfected with an human RhoB expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 6:

293T transfected with an human RhoB expression vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Lane 7:

293T transfected with an human RhoC expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 8:

293T transfected with an human RhoC expression vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Observed band size: 22 kDa,17 kDa

false

Exposure time: 1s