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AB53743

Anti-RHOB antibody [2916C2a]

3

(1 Review)

|

(4 Publications)

Mouse Monoclonal RHOB antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RHOB.

View Alternative Names

ARH6, ARHB, RHOB, Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6

1 Images
Western blot - Anti-RHOB antibody [2916C2a] (AB53743)
  • WB

Unknown

Western blot - Anti-RHOB antibody [2916C2a] (AB53743)

The band on the Wetstern blot does not correspond to the predicted size, as the recombinant protein was used as the test antigen

All lanes:

Western blot - Anti-RHOB antibody [2916C2a] (ab53743)

All lanes:

immunized recombinant protein

Predicted band size: 22 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2916C2a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RHOB. The exact immunogen used to generate this antibody is proprietary information.

P62745

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from culture supernatant
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHOB also known as Ras Homolog Family Member B is a small GTPase with a molecular mass of approximately 21 kDa. It acts as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. RHOB regulates various cellular functions including cytoskeletal organization and cell signaling. It is expressed in different human tissues with increased levels in the placenta lungs and testes.
Biological function summary

RHOB influences cell shape and motility by modulating actin architecture. It is part of the Rho GTPase family which impacts diverse cellular processes such as cell proliferation survival and vesicle trafficking. RHOB operates within protein complexes that contribute to its regulatory functions. It has an important role in controlling endosome dynamics and influences the signaling pathways that respond to environmental cues.

Pathways

By affecting cytoskeletal remodeling RHOB plays a role in the Rho signaling pathway. It interacts with related proteins like RHOA and CDC42 facilitating the coordination of these pathways. Additionally RHOB is involved in the integrin signaling pathway which impacts cell adhesion and migration processes. These pathways are significant for understanding how cells move and communicate in response to external stimuli.

RHOB has implications in cancer development and progression. Aberrant expression or mutation of RHOB can correlate with tumor suppression or oncogenesis depending on the cellular context. It is associated with proteins such as AKT and PTEN which modulate cell survival and growth signals which are important in cancer biology. Furthermore RHOB shows involvement in cardiovascular diseases where it influences vascular remodeling processes through interactions with proteins like VEGFR2.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
See full target information RHOB
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:8253-8261 PubMed32982421

2020

LncRNA GAS5 Regulates Osteosarcoma Cell Proliferation, Migration, and Invasion by Regulating RHOB via Sponging miR-663a.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Yao,Xianan Li,Yi Luo,Xuezheng Xu,Jianfan Liu,Jie Bu

Experimental and therapeutic medicine 18:5072-5077 PubMed31819770

2019

Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Liu,Jian Zhang,Pingping Zhou,Hao Sun,Martha Katsarou,Nikolaos Drakoulis

Journal of virology 83:10176-86 PubMed19625394

2009

Rho GTPases modulate entry of Ebola virus and vesicular stomatitis virus pseudotyped vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrina Quinn,Melinda A Brindley,Melodie L Weller,Nikola Kaludov,Andrew Kondratowicz,Catherine L Hunt,Patrick L Sinn,Paul B McCray,Colleen S Stein,Beverly L Davidson,Ramon Flick,Robert Mandell,William Staplin,Wendy Maury,John A Chiorini

British journal of cancer 99:1322-9 PubMed18827818

2008

NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer.

Applications

WB

Species

Human

D Murray,G Horgan,P Macmathuna,P Doran
View all publications
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Product promise

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