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AB150408

Anti-RHOBTB1 antibody [EPR8990]

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(1 Publication)

Rabbit Recombinant Monoclonal RHOBTB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RHOBTB1 antibody [EPR8990] (AB150408)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

Immunofluorescent analysis of JAR cells labelling RHOBTB1 with ab150408 at a dilution of 1/250.

Western blot - Anti-RHOBTB1 antibody [EPR8990] (AB150408)
  • WB

Unknown

Western blot - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

All lanes:

Western blot - Anti-RHOBTB1 antibody [EPR8990] (ab150408) at 1/1000 dilution

Lane 1:

Human stomach lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Lane 3:

Human placenta lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

false

  • Carrier free

    Anti-RHOBTB1 antibody [EPR8990] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8990

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHOBTB1 also known as Rho-related BTB domain-containing protein 1 is a member of the Rho GTPase family. It has a molecular mass of approximately 82 kDa. This protein is mainly expressed in the brain and testis with lower levels found in other tissues. RHOBTB1 functions by acting as a molecular switch that cycles between an active GTP-bound state and an inactive GDP-bound state which regulates various cellular processes.
Biological function summary

RHOBTB1 is involved in the regulation of cytoskeletal dynamics which affects cell shape motility and possibly cell division. It is also implicated in intracellular signaling processes that control cell morphology. RHOBTB1 does not function alone; it interacts with other proteins possibly forming part of larger protein complexes that further expand its role in cellular activities.

Pathways

RHOBTB1 plays a role in signaling pathways that include the Rho GTPase cycle and cellular processes related to cytoskeletal reorganization. In these pathways RHOBTB1 interacts with proteins such as CDC42 and RAC1 which are essential for actin filament polymerization and cellular movement. These interactions help facilitate normal cellular dynamics and responses to extracellular signals.

Mutations or dysregulation of RHOBTB1 have been linked to neurological conditions and certain cancers. For example RHOBTB1 abnormalities have been associated with an increased risk of glioblastoma where it might interact with tumor suppressor proteins like p53. Additionally RHOBTB1's role in vacuole formation has implications in lysosomal storage diseases connecting it to other lysosomal proteins involved in these processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information RHOBTB1

Alternative Names

KIAA0740, RHOBTB1, Rho-related BTB domain-containing protein 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 147:65 PubMed38557897

2024

Landscape of brain myeloid cell transcriptome along the spatiotemporal progression of Alzheimer's disease reveals distinct sequential responses to Aβ and tau.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid Wachter,Maya E Woodbury,Sylvia Lombardo,Aicha Abdourahman,Carolin Wuest,Emily McGlame,Timothy Pastika,Joseph Tamm,Nandini Romanul,Kiran Yanamandra,Rachel Bennett,Gen Lin,Taekyung Kwon,Fan Liao,Corinna Klein,Yelena Grinberg,Methasit Jaisa-Aad,Huan Li,Matthew P Frosch,Markus P Kummer,Sudeshna Das,Tammy Dellovade,Eric H Karran,Xavier Langlois,Janina S Ried,Alberto Serrano-Pozo,Robert V Talanian,Knut Biber,Bradley T Hyman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com