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AB7253

Rhodamine Anti-Fluorescein antibody

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(1 Publication)

Goat Polyclonal Fluorescein antibody - conjugated to Rhodamine. Suitable for Flow Cyt, IM and reacts with Chemical samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Fluorescein.

View Alternative Names

FI, fluorescein isothyocianate, fluorescyl

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Rhodamine

Excitation/Emission

Ex: 550nm, Em: 570nm

Carrier free

No

Applications

IM, Flow Cyt

applications

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Alternative Products

Primary Antibodies

AB206506

Anti-Fluorescein antibody [4-4-20 (enhanced)]

Recombinant

  • 0
  • 0
Primary Antibodies

AB206508

Anti-Fluorescein antibody [4-4-20 (enhanced)]

Recombinant

  • 0
  • 0
Primary Antibodies

AB206509

Anti-Fluorescein antibody [4-4-20 (enhanced)]

Recombinant

  • 0
  • 0

Complementary Products

Primary Antibodies

AB19224

Anti-FITC antibody

  • 0
  • 0
Primary Antibodies

AB9093

Anti-Rhodamine antibody [11H10]

Dot Blot - Anti-Rhodamine antibody [11H10] (AB9093)

  • 1
  • 1
Primary Antibodies

AB6653

Texas Red ® Anti-Biotin antibody

Western blot - Texas Red ® Anti-Biotin antibody (AB6653)

  • 0
  • 0
Primary Antibodies

AB6215

Biotin Anti-Fluorescein antibody [BT.6A4]

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IM" : {"fullname" : "Immunomicroscopy", "shortname":"IM"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IM-species-checked": "guaranteed", "IM-species-dilution-info": "", "IM-species-notes": "<p></p>" } } }
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Product details

Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Goat IgG.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Fluorescein Antibody Conjugated to Rhodamine was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fluorescein alternatively known as fluoresceine or resorcinol phthalein is a synthetic compound with a molecular mass of approximately 332.31 g/mol. This organic dye is widely used in science and medicine due to its intense fluorescence. Typically fluorescein is expressed in environments where a fluorescent marker is valuable such as in imaging applications. As a small highly fluorescent molecule it can easily bind to different biomolecules. In the context of the commercial world you may encounter the term anti-fluorescein referring to antibodies developed specifically against fluorescein to enhance detection or removal from samples.
Biological function summary

Fluorescein functions as a non-invasive marker that can bind to proteins such as biotin without altering their biological properties. This feature of fluorescein allows it to participate in biotin fluorescence systems aiding in the visualization of proteins or nucleic acids. In many experimental setups researchers utilize biotin-fluorescence or fluorescent biotin complexes to map biological activities or protein localization. Despite not being a natural part of cellular complexes its versatility and binding compatibility make it a popular choice in assays and diagnostic processes.

Pathways

Researchers use fluorescein in a variety of systems such as the biotin-fluorophore pathway helping track the activity and movement of biomolecules within cells. While not inherently a part of natural biological pathways it plays an important annotative role in studying processes involving proteins like avidin or streptavidin. These proteins can strongly bind to biotin and by extension fluorescently labeled biotin enabling the visualization of pathways and interactions that would otherwise be difficult to study.

Fluorescein assists in the diagnosis and study of conditions involving retinal blood flow such as diabetic retinopathy or macular degeneration. During fluorophotometry or fluorescein angiography it helps visualize blood flow or tissue perfusion in disorders. When performing these diagnostics clinicians may use anti-fluorescein antibodies to neutralize excess fluorescein preventing unwanted distribution and ensuring higher resolution results. Although not directly linked to specific proteins through diseases its utility in diagnostics allows elucidation of pathologies and conditions related to eye health and vascular systems.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Fluorescein
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunological methods 433:6-16 PubMed26921630

2016

Development of a custom pentaplex sandwich immunoassay using Protein-G coupled beads for the Luminex® xMAP® platform.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline U McDonald,Julia Ekeruche-Makinde,Mei M Ho,John S Tregoning,Omodele Ashiru
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Product promise

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