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AB190307

Anti-Rhodopsin antibody [A531]

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(5 Publications)

Mouse Monoclonal Rhodopsin antibody. Suitable for WB, ICC/IF and reacts with Cow, Pig samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Rhodopsin.

View Alternative Names

Rhodopsin, RHO

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Rhodopsin antibody [A531] (AB190307)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rhodopsin antibody [A531] (AB190307)

Immunofluorescent analysis of pig retinal tissue labeling Rhodopsin with ab190307 at 1/1000 dilution (green) and counterstained with rabbit polyclonal antibody to neurofilament (red) and DNA (blue).

Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).

Western blot - Anti-Rhodopsin antibody [A531] (AB190307)
  • WB

Supplier Data

Western blot - Anti-Rhodopsin antibody [A531] (AB190307)

Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin.

Note, due to the highly hydrophobic nature of rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.

All lanes:

Western blot - Anti-Rhodopsin antibody [A531] (ab190307) at 1/5000 dilution

All lanes:

Bovine retinal extract

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A531

Isotype

IgG1

Carrier free

No

Reacts with

Cow, Pig

Applications

ICC/IF, WB

applications

Immunogen

Native Full Length Protein corresponding to Cow Rhodopsin.

P02699

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rhodopsin also known as visual purple is a protein found in the photoreceptor cells of the retina. It has a mass of approximately 39 kDa. This protein acts as a light-sensitive receptor playing an essential role in the function of rod cells. Rhodopsin is expressed predominantly in the rod outer segment where it transduces light signals. The crystallized form of this receptor referred to as PDB ID: 1D4 has helped researchers study its structure in detail.
Biological function summary

Rhodopsin enables night vision by absorbing photons and activating a G-protein-coupled receptor (GPCR) cascade. Rhodopsin is an integral part of a protein complex within the phototransduction pathway where it works closely with transducin to amplify the visual signal. It initiates the conversion of the photon into an electrical signal. Rhodopsin's role is triggering a structural change in response to light converting from its inactive form to a signaling state.

Pathways

Rhodopsin is deeply involved in the phototransduction and retinoid cycle pathways. Once it absorbs light the transducin it binds causes the activation of a cascade that ultimately results in hyperpolarization of the rod cells. This network of interactions also involves arrestin and recoverin which work together to regulate rhodopsin activity and signal termination. The process ensures precise response and recovery of the visual signal in low-light conditions.

Rhodopsin mutations and malfunctions connect to retinitis pigmentosa and congenital stationary night blindness. Mutations can lead to improper folding or misregulation of the protein impacting its function. Visual cycle proteins like RPE65 are often affected alongside rhodopsin in retinitis pigmentosa. These associations stress rhodopsin's importance in maintaining healthy retinal function and its link to eye diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed : 10926528, PubMed : 11972040, PubMed : 12044163, PubMed : 16586416, PubMed : 16908857, PubMed : 17060607, PubMed : 17449675, PubMed : 18818650, PubMed : 21389983, PubMed : 22198838, PubMed : 23579341, PubMed : 25205354, PubMed : 27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed : 1396673, PubMed : 15111114).
See full target information Rhodopsin
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:19457 PubMed39169055

2024

Selective nanosecond laser removal of retinal pigment epithelium for cell therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Van Phuc Nguyen,Athanasios J Karoukis,Justin Hu,Zhuying Wei,Dongshan Yang,Abigail T Fahim,Xueding Wang,Yannis M Paulus

Antioxidants (Basel, Switzerland) 13: PubMed38397799

2024

Piceid Octanoate Protects Retinal Cells against Oxidative Damage by Regulating the Sirtuin 1/Poly-ADP-Ribose Polymerase 1 Axis In Vitro and in rd10 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Seyed Mohamadmehdi Moshtaghion,Estefanía Caballano-Infantes,Álvaro Plaza Reyes,Lourdes Valdés-Sánchez,Patricia Gallego Fernández,Berta de la Cerda,Maurizio S Riga,Manuel Álvarez-Dolado,Pablo Peñalver,Juan C Morales,Francisco J Díaz-Corrales

Micromachines 12: PubMed34945319

2021

Biocompatibility Study of a Commercial Printed Circuit Board for Biomedical Applications: Lab-on-PCB for Organotypic Retina Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús David Urbano-Gámez,Lourdes Valdés-Sánchez,Carmen Aracil,Berta de la Cerda,Francisco Perdigones,Álvaro Plaza Reyes,Francisco J Díaz-Corrales,Isabel Relimpio López,José Manuel Quero

Molecular medicine (Cambridge, Mass.) 26:1 PubMed31892304

2019

Retinal pigment epithelium degeneration caused by aggregation of PRPF31 and the role of HSP70 family of proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lourdes Valdés-Sánchez,Sofia M Calado,Berta de la Cerda,Ana Aramburu,Ana Belén García-Delgado,Simone Massalini,Adoración Montero-Sánchez,Vaibhav Bhatia,Eduardo Rodríguez-Bocanegra,Andrea Diez-Lloret,Daniel Rodríguez-Martínez,Christina Chakarova,Shom S Bhattacharya,Francisco J Díaz-Corrales

International journal of molecular sciences 19: PubMed30366444

2018

IL-18 and S100A12 Are Upregulated in Experimental Central Retinal Vein Occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Lasse Jørgensen Cehofski,Anders Kruse,Svend Kirkeby,Alexander Nørgård Alsing,Jonas Ellegaard Nielsen,Kentaro Kojima,Bent Honoré,Henrik Vorum
View all publications
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