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AB230692

Anti-Rhodopsin antibody [B630]

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(3 Publications)

Mouse Monoclonal Rhodopsin antibody. Suitable for IHC-P, WB and reacts with Pig, Human, Cow samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Rhodopsin.

View Alternative Names

OPN2, RHO, Rhodopsin, Opsin-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (AB230692)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (AB230692)

Formalin-fixed, paraffin-embedded human retinal tissue stained for Rhodopsin with ab230692 at 10 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (AB230692)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [B630] (AB230692)

Formalin-fixed, paraffin-embedded pig retinal tissue stained for Rhodopsin with ab230692 (green) at 10 μg/ml in immunohistochemical analysis.

Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).

Western blot - Anti-Rhodopsin antibody [B630] (AB230692)
  • WB

Supplier Data

Western blot - Anti-Rhodopsin antibody [B630] (AB230692)

Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin.

All lanes:

Western blot - Anti-Rhodopsin antibody [B630] (ab230692) at 1/5000 dilution

All lanes:

bovine retinal extracts

Predicted band size: 38 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B630

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Cow, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Cow Rhodopsin.

P02699

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p><strong>Note:</strong> Due to the highly hydrophobic nature of rhodopsin, it's important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.</p>" }, "Pig": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Protein G purified from tissue culture supernatant.
Storage buffer
Preservative: 0.07% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rhodopsin also known as visual purple is a protein found in the photoreceptor cells of the retina. It has a mass of approximately 39 kDa. This protein acts as a light-sensitive receptor playing an essential role in the function of rod cells. Rhodopsin is expressed predominantly in the rod outer segment where it transduces light signals. The crystallized form of this receptor referred to as PDB ID: 1D4 has helped researchers study its structure in detail.
Biological function summary

Rhodopsin enables night vision by absorbing photons and activating a G-protein-coupled receptor (GPCR) cascade. Rhodopsin is an integral part of a protein complex within the phototransduction pathway where it works closely with transducin to amplify the visual signal. It initiates the conversion of the photon into an electrical signal. Rhodopsin's role is triggering a structural change in response to light converting from its inactive form to a signaling state.

Pathways

Rhodopsin is deeply involved in the phototransduction and retinoid cycle pathways. Once it absorbs light the transducin it binds causes the activation of a cascade that ultimately results in hyperpolarization of the rod cells. This network of interactions also involves arrestin and recoverin which work together to regulate rhodopsin activity and signal termination. The process ensures precise response and recovery of the visual signal in low-light conditions.

Rhodopsin mutations and malfunctions connect to retinitis pigmentosa and congenital stationary night blindness. Mutations can lead to improper folding or misregulation of the protein impacting its function. Visual cycle proteins like RPE65 are often affected alongside rhodopsin in retinitis pigmentosa. These associations stress rhodopsin's importance in maintaining healthy retinal function and its link to eye diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Photoreceptor required for image-forming vision at low light intensity (PubMed : 7846071, PubMed : 8107847). Required for photoreceptor cell viability after birth (PubMed : 12566452, PubMed : 2215617). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed : 26200343, PubMed : 28524165, PubMed : 28753425, PubMed : 8107847). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed : 26200343, PubMed : 28524165).
See full target information RHO
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade9459 PubMed36662852

2023

Inflammation of the retinal pigment epithelium drives early-onset photoreceptor degeneration in -associated retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Maria E Mercau,Yemsratch T Akalu,Francesca Mazzoni,Gavin Gyimesi,Emily J Alberto,Yong Kong,Brian P Hafler,Silvia C Finnemann,Carla V Rothlin,Sourav Ghosh

Scientific reports 12:21825 PubMed36528753

2022

Periportal steatosis in mice affects distinct parameters of pericentral drug metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Albadry,Sebastian Höpfl,Nadia Ehteshamzad,Matthias König,Michael Böttcher,Jasna Neumann,Amelie Lupp,Olaf Dirsch,Nicole Radde,Bruno Christ,Madlen Christ,Lars Ole Schwen,Hendrik Laue,Robert Klopfleisch,Uta Dahmen

International journal of molecular sciences 23: PubMed35887093

2022

PEDF Deletion Induces Senescence and Defects in Phagocytosis in the RPE.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan T Rebustini,Susan E Crawford,S Patricia Becerra
View all publications
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