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AB101349

Anti-RhoF antibody

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(5 Publications)

Rabbit Polyclonal RHOF antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RHOF aa 1-200.

View Alternative Names

ARHF, RIF, RHOF, Rho-related GTP-binding protein RhoF, Rho family GTPase Rif, Rho in filopodia

1 Images
Western blot - Anti-RhoF antibody (AB101349)
  • WB

Unknown

Western blot - Anti-RhoF antibody (AB101349)

12% SDS PAGE

All lanes:

Western blot - Anti-RhoF antibody (ab101349) at 1/500 dilution

All lanes:

U87-MG whole cell lysate at 30 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RHOF aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9HBH0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RhoF also known as Rif or Rho-related GTP-binding protein RhoF is a member of the Rho family of small GTPases. It has a molecular mass of around 21 kDa. RhoF acts as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. It is known to regulate the organization of the actin cytoskeleton. RhoF expression is quite marked in tissues like the brain and testis where it carries out its functions by interacting with various effector proteins.
Biological function summary

RhoF plays a role in organizing the actin cytoskeleton and is not found as part of a larger protein complex. It triggers the formation of filopodia and other actin-associating structures influencing cell shape and movement. RhoF is central to processes that depend on dynamic actin remodeling such as cell migration adhesion and morphogenesis. Through these interactions it impacts cell functionality in various cellular contexts.

Pathways

Its activity is associated with the regulation of actin cytoskeleton pathways. RhoF interacts with proteins like Cdc42 and Rac1 members of the same Rho GTPase family which also participate in these pathways. It contributes to the signaling networks that govern cytoskeletal rearrangements and influences cellular dynamics. This integration into these pathways allows RhoF to influence downstream effector pathways assisting cells in responding to extracellular cues.

RhoF's role in actin cytoskeleton regulation links it to conditions involving cytoskeletal dysfunction such as certain types of cancers and developmental disorders. In cancer its interaction with related proteins like Rac1 may influence tumor cell migration and metastasis. In developmental disorders improper regulation of RhoF and its signaling pathways can result in abnormal cell morphology and motility affecting organ development and function. These associations make RhoF a potential target for therapeutic interventions in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
See full target information RHOF
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 12:32 PubMed39462429

2024

RHOF promotes Snail1 lactylation by enhancing PKM2-mediated glycolysis to induce pancreatic cancer cell endothelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Yanmin Yi,Han Liu,Jianwei Xu,Shuhai Chen,Dong Wu,Lei Wang,Feng Li

The Journal of biological chemistry 299:105248 PubMed37703992

2023

Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Michiru Nishita,Koki Kamizaki,Kyoka Hoshi,Kana Aruga,Ikumi Nishikaku,Hiroshi Shibuya,Kunio Matsumoto,Yasuhiro Minami

Cell reports 35:109102 PubMed33979620

2021

RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Heib,Heike M Hermanns,Georgi Manukjan,Maximilian Englert,Charly Kusch,Isabelle Carlotta Becker,Annika Gerber,Lou Martha Wackerbarth,Philipp Burkard,Thomas Dandekar,Johannes Balkenhol,Daniel Jahn,Sarah Beck,Mara Meub,Sebastian Dütting,Christian Stigloher,Markus Sauer,Deya Cherpokova,Harald Schulze,Cord Brakebusch,Bernhard Nieswandt,Zoltan Nagy,Irina Pleines

Cell transplantation 28:1271-1278 PubMed31250656

2019

Metabolic Syndrome Induces Release of Smaller Extracellular Vesicles from Porcine Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sabena M Conley,John E Shook,Xiang-Yang Zhu,Alfonso Eirin,Kyra L Jordan,John R Woollard,Busra Isik,LaTonya J Hickson,Amrutesh S Puranik,Lilach O Lerman

Cell death & disease 8:e3129 PubMed29048402

2017

miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis.

Applications

WB, IHC-P

Species

Unspecified reactive species, Unspecified reactive species

Rui-Meng Yang,Ming Zhan,Sun-Wang Xu,Man-Mei Long,Lin-Hua Yang,Wei Chen,Shuai Huang,Qiang Liu,Jun Zhou,Jun Zhu,Jian Wang
View all publications
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