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Mouse Recombinant Monoclonal RHOG antibody. Suitable for ICC/IF, IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-RHOG antibody [1F3-RHOG] (AB254147), expandable thumbnail
  • Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (AB254147), expandable thumbnail
  • Western blot - Anti-RHOG antibody [1F3-RHOG] (AB254147), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RHOG antibody [1F3-RHOG] (AB254147), expandable thumbnail
  • Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (AB254147), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Plays a role in immunological synaptic F-actin density and architecture organization (PubMed:33513601). Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity (PubMed:33513601). Required for the formation of membrane ruffles during macropinocytosis (PubMed:15133129). Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes (PubMed:17875742). Binds phospholipids in an activation-dependent manner; thereby acting as an anchor for other proteins to the plasma membrane (PM) (PubMed:33513601). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells (PubMed:33513601). Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D (PubMed:33513601). Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells (PubMed:33513601). (Microbial infection) In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.

Alternative names

ARHG, RHOG, Rho-related GTP-binding protein RhoG

Recommended products

Mouse Recombinant Monoclonal RHOG antibody. Suitable for ICC/IF, IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
1F3-RHOG
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RHOG also known as ras homolog gene family member G is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rho family which plays a role in various cellular functions. RHOG is highly expressed in hematopoietic cells with significant presence in macrophages and neutrophils. This protein is also found in various tissues where it assists in cell movement and cytoskeletal rearrangements.

Biological function summary

RHOG regulates cell shape and motility. It is involved in forming membrane protrusions such as filopodia and lamellipodia. RHOG can act as a part of a protein complex with other GTPases. It controls actin cytoskeleton dynamics by activating pathways needed for cell migration and chemotaxis. This protein significantly aids immune cell function during immune surveillance and inflammatory responses.

Pathways

RHOG interacts with diverse signaling cascades. It activates the Rac1 pathway influencing cellular migration and growth processes. RHOG collaborates with proteins like PI3-kinase in these pathways contributing to the regulation of cytoskeletal organization. RHOG involvement in these signaling networks ensures cells respond aptly to external cues maintaining cellular integrity and function.

Associated diseases and disorders

RHOG is implicated in cancer and chronic inflammatory diseases. Abnormal RHOG expression or activity can lead to excessive cell movement and invasion often linked to cancer metastasis. Additionally it relates to chronic inflammatory responses where dysregulation may exacerbate disorders such as rheumatoid arthritis. In these conditions RHOG interacts with proteins like RhoA to mediate changes in cell behavior that drive disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    False colour image of Western blot: Anti-RHOG antibody [1F3-RHOG] staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab254147 was shown to bind specifically to RHOG. A band was observed at 18 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in RHOG knockout cell line Human RHOG knockout HEK-293T cell line ab266753 (knockout cell lysate Human RHOG knockout HEK-293T cell lysate ab263328). To generate this image, wild-type and RHOG knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.

    All lanes: Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: RHOG knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human RHOG knockout HEK-293T cell line (Human RHOG knockout HEK-293T cell line ab266753)

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: SH-SY5Y cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Observed band size: 18 kDa

  • Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    RHOG was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte), whole cell lysate with ab254147 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254147 at 1/1000 dilution. Mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate 10μg.

    Lane 2: ab254147 IP in Jurkat whole cell lysate.

    Lane 3: Mouse monoclonal IgG2b instead of ab254147 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    Predicted band size: 21 kDa

    Observed band size: 18 kDa

  • Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID:18505794).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 2: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 4: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labelling RHOG with ab254147 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 goat anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cells. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 anti-beta Tubulin rabbit monoclonal antibody at a 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution was used to counterstain tubulin (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab254147 at a 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/500 dilution.

    Negative control 2: Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution.

  • Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    RHOG was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate with abab254147 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254147 at 1/1000 dilution. Mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/1000 dilution.

    Lane 1: J774A.1 whole cell lysate 10μg.

    Lane 2: ab254147 IP in J774A.1 whole cell lysate.

    Lane 3: Mouse monoclonal IgG2b instead of ab254147 in J774A.1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    Predicted band size: 21 kDa

    Observed band size: 18 kDa

  • Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-RHOG antibody [1F3-RHOG] (ab254147) at 1/1000 dilution

    Lane 1: Mouse bone marrow tissue lysate at 20 µg

    Lane 2: Rat thymus tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RHOG antibody [1F3-RHOG] (ab254147), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RHOG antibody [1F3-RHOG] (ab254147)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 cells labelling RHOG with ab254147 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 goat anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in J774A.1 cells. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 anti-beta Tubulin rabbit monoclonal antibody at a 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution was used to counterstain tubulin (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab254147 at a 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/500 dilution.

    Negative control 2: Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 at a 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at a 1/1000 dilution.

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Product protocols

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