AB118507

Anti-RHOH antibody

Name: Anti-RHOH antibody (ab118507) | Abcam
Brand: Abcam Limited
SKU: AB118507
Price: 630 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RHOH antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RHOH conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ARHH, TTF, RHOH, Rho-related GTP-binding protein RhoH, GTP-binding protein TTF, Translocation three four protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (AB118507)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (AB118507)

ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human Small Intestine tissue.

Immunocytochemistry/ Immunofluorescence - Anti-RHOH antibody (AB118507)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RHOH antibody (AB118507)

Immunofluorescence analysis of A549 cells, using ab118507 at 1/100. The picture on the right is treated with the synthesized peptide.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (AB118507)

ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human colon tissue.

Unknown

Western blot - Anti-RHOH antibody (AB118507)

All lanes:

Western blot - Anti-RHOH antibody (ab118507) at 1/500 dilution

Lane 1:

HT-29 extract without the synthesized peptide.

Lane 2:

HT-29 extract with the synthesized peptide.

Predicted band size: 20 kDa,21 kDa,25 kDa,38 kDa,42 kDa,48 kDa,61 kDa,62 kDa,66 kDa,68 kDa,93 kDa

Observed band size: 116 kDa,117 kDa,25 kDa,55 kDa,56 kDa,61 kDa,82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RHOH conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15669

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store undiluted

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHOH also known as Rho-related GTP-binding protein RhoH is a small GTPase belonging to the Ras superfamily. It has a molecular weight of approximately 21 kDa. This protein is expressed mainly in hematopoietic cells such as lymphocytes where it plays a mechanical role in modulating cytoskeletal organization by regulating actin filament structures. Unlike other Rho GTPases RHOH lacks the intrinsic GTPase activity which means it stays in the GTP-bound form and functions as a constitutive signaling molecule.

Biological function summary

RHOH contributes to signal transduction pathways essential for immune function. It is involved in T-cell receptor signaling and acts as a negative regulator of the RhoA and Rac1 pathways. RHOH forms complexes with other signaling molecules which impacts T-cell development and activation linking cytoskeletal dynamics and membrane trafficking to signaling processes.

Pathways

Researchers identified RHOH as significant in the NF-kB signaling pathway and T-cell antigen receptor (TCR) signaling. Through these pathways RHOH interacts with proteins like ZAP-70 and Lck influencing the downstream signaling events important for immune cell activation and response. Consequently RHOH modulates interactions that are vital for immune system adaptations and activities.

RHOH mutations and dysregulation connect to specific forms of leukemias and lymphomas. Aberrant expression or function of RHOH correlates with chronic lymphocytic leukemia (CLL) wherein altered signaling contributes to cancer pathogenesis. Through these disease states RHOH is linked with proteins such as Syk which also play roles in the altered signaling pathways characteristic of these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.

See full target information RHOH

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 71:36-42 PubMed39976915

2025

Notch/IL33/ST2 signaling was involved in the maintenance of intestinal epithelial barrier through regulating tight junction after LPS stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanling Zhang,Chao Xu,Fang Li,Guoqing Chen

Laboratory investigation; a journal of technical methods and pathology 102:782-793 PubMed35149775

2022

DNA methyltransferase 1 (DNMT1) suppresses mitophagy and aggravates heart failure via the microRNA-152-3p/ETS1/RhoH axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuojun Deng,Jiaqi Yao,Na Xiao,Yu Han,Xuan Wu,Caizhe Ci,Ke Chen,Xiaoyong Geng

International journal of clinical and experimental 11:5830-5839 PubMed31949669

2018

miR-3646 promotes vascular inflammation and augments vascular smooth muscle cell proliferation and migration in progression of coronary artery disease by directly targeting RHOH.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Kang,Simin Cao,Zheng Ji,Yu Zhang,Shuxian Sun,Xiaoming Shang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RHOH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (AB118507)

Immunocytochemistry/ Immunofluorescence - Anti-RHOH antibody (AB118507)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOH antibody (AB118507)

Western blot - Anti-RHOH antibody (AB118507)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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