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AB63799

Anti-Rhp51 antibody

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(5 Publications)

Rabbit Polyclonal Rad51 antibody. Suitable for IP, WB, ICC/IF and reacts with Schizosaccharomyces pombe samples. Cited in 5 publications.

View Alternative Names

DNA repair protein rhp51, RAD51 homolog, Recombinase Rhp51

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Rhp51 antibody (AB63799)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rhp51 antibody (AB63799)

ab63799 (1/500) staining Rhp51 in S. pombe cells without or with MMS (0.025%) treatment for 1 hour by ICC/IF (Immunocytochemistry/immunofluorescence).

Western blot - Anti-Rhp51 antibody (AB63799)
  • WB

Unknown

Western blot - Anti-Rhp51 antibody (AB63799)

All lanes:

Western blot - Anti-Rhp51 antibody (ab63799) at 1/2000 dilution

Lane 1:

Molecular weight markers

Lane 2:

Whole cell extract of Schizosaccharomyces pombe wild-type strain

Lane 3:

Whole cell extract of Schizosaccharomyces pombe Rad51 deletion strain

Predicted band size: 40 kDa

Observed band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Schizosaccharomyces pombe

Applications

IP, ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Schizosaccharomyces pombe": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.09% Sodium azide Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rhp51 also known in some organisms as Rad51 is a protein involved in homologous recombination a process essential for DNA repair. This protein has a molecular mass of approximately 37 kDa. Rhp51 functions by facilitating the search for homology and strand pairing between homologous DNA sequences. It is expressed in various tissues where cells undergo active division making it especially prominent in rapidly proliferating tissues and germ cells.
Biological function summary

Rhp51 facilitates critical steps of homologous recombination through its interactions with DNA and other repair proteins. Rhp51 is part of a protein complex that includes Rad52 which assists Rhp51 in its function. Through its role in DNA repair Rhp51 maintains genome integrity prevents mutations and supports proper chromosomal segregation during cell division. It interacts with both the single-stranded DNA and other DNA repair proteins to ensure accurate repair processes within the cell.

Pathways

Rhp51 is an integral component of the DNA damage response and repair pathways specifically the homologous recombination repair pathway. It interacts closely with proteins like BRCA2 which mediates the loading of Rhp51 onto DNA. In addition Rhp51 collaborates closely with Rad54 a protein that aids in the resolution of junctions formed during recombination. These interactions are critical for repairing double-strand breaks a common type of DNA damage.

Mutations or dysfunctions in Rhp51 can lead to genomic instability contributing to an increased risk of cancer development particularly breast and ovarian cancers. Its role in cancer is tightly connected with BRCA1 and BRCA2 proteins whose mutations are well-known cancer risk factors. Moreover improper function of Rhp51 may result in Bloom syndrome a rarer disorder characterized by genomic instability where Rhp51's coaction with the BLM protein is affected leading to defective DNA repair mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rhp51
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40737093

2025

S-phase checkpoint protects from aberrant replication fork processing and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Núñez-Martín,Lucy S Drury,María I Martínez-Jiménez,Luis Blanco,John F X Diffley,Andrés Aguilera,Belén Gómez-González

Nucleic acids research 49:6832-6848 PubMed34157114

2021

Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Jakub Muraszko,Karol Kramarz,Bilge Argunhan,Kentaro Ito,Gabriela Baranowska,Yumiko Kurokawa,Yasuto Murayama,Hideo Tsubouchi,Sarah Lambert,Hiroshi Iwasaki,Dorota Dziadkowiec

Methods in molecular biology (Clifton, N.J.) 2267:191-205 PubMed33786793

2021

Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Rohana Ramalingam,Matthew J O'Connell

Nature communications 11:5643 PubMed33159083

2020

The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal.

Applications

Unspecified application

Species

Unspecified reactive species

Karol Kramarz,Kamila Schirmeisen,Virginie Boucherit,Anissia Ait Saada,Claire Lovo,Benoit Palancade,Catherine Freudenreich,Sarah A E Lambert

Cell reports 30:3312-3322.e3 PubMed32160539

2020

Telomerase Repairs Collapsed Replication Forks at Telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Samah Matmati,Sarah Lambert,Vincent Géli,Stéphane Coulon
View all publications
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