AB92537

Anti-RIAM antibody [EPR2806]

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal RIAM antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

PREL1, RARP1, RIAM, APBB1IP, Amyloid beta A4 precursor protein-binding family B member 1-interacting protein, APBB1-interacting protein 1, Proline-rich EVH1 ligand 1, Proline-rich protein 73, Rap1-GTP-interacting adapter molecule, Retinoic acid-responsive proline-rich protein 1, PREL-1, RARP-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] (AB92537)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] (AB92537)

ab92537, at 1/100 dilution, staining RIAM in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RIAM antibody [EPR2806] (AB92537)

All lanes:

Western blot - Anti-RIAM antibody [EPR2806] (ab92537) at 1/2000 dilution

Lane 1:

Human Lymph node lysate at 10 µg

Lane 2:

Fetal brain lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

HL-60 + retinoic acid cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2806

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIAM also known as Rap1-GTP-interacting adaptor molecule is a protein involved in intracellular signaling. RIAM has a molecular weight of approximately 76 kDa. It is expressed in various tissues with a significant presence in hematopoietic cells and endothelial cells. This protein plays a part in connecting cell membrane molecules with actin cytoskeleton therefore promoting cellular adhesion.

Biological function summary

RIAM is essential for the integrin-mediated adhesion processes. It functions as a critical adaptor forming complexes with talin and other signaling proteins which facilitate the activation of integrins. Through these actions RIAM contributes to cell migration immune responses and platelet aggregation. Its interactions influence cellular adhesion and communication pivotal for maintaining tissue structure and response to injuries.

Pathways

RIAM is a vital component in the signaling pathways involving the small GTPase Rap1 which regulates integrins and their effect on cell adhesion. Particularly RIAM serves as a bridge between Rap1 and talin facilitating integrin activation. This process plays an essential role in the focal adhesion pathway where proteins like Kindlin also collaborate to modulate cellular adhesion and movement impacting various physiological processes.

RIAM has connections with immune dysfunction and cardiovascular diseases. For example abnormalities in RIAM-mediated integrin activation can lead to improper immune cell trafficking and related immunodeficiencies. In cardiovascular contexts defective RIAM activity might impair endothelial cell function and contribute to vascular diseases. In these scenarios proteins like talin are closely associated with RIAM often sharing roles in the pathogenesis of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.

See full target information APBB1IP

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadt6083 PubMed40911671

2025

Mechanosensitive biochemical imprinting of the talin interaction with DLC1 regulates RhoA activity and cardiomyocyte remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Marhuenda,Ioannis Xanthis,Poppy O Smith,Aishwarya Prakash,Till Kallem,Pragati Pandey,Darren Graham Samuel Wilson,Amar Azad,Megan Richter,Davor Pavlovic,Katja Gehmlich,Giuseppe Faggian,Pamela Swiatlowska,Elisabeth Ehler,James Levitt,Simon P Poland,Simon Ameer-Beg,Benjamin T Goult,Thomas Iskratsch

Frontiers in pharmacology 16:1535182 PubMed40183091

2025

Inflammation and platelet hyperresponsiveness in coronary artery disease and the influence of Talin-1/αIIbβ3-mediated bidirectional signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Shi,Jiaming Gao,Yehao Zhang,Min Zhan,Zhanfei Tan,Peili Wang,Jianhua Fu,Jianxun Liu

Journal of cell science 135: PubMed34859816

2022

Inhibition of minor intron splicing reduces Na+ and Ca2+ channel expression and function in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Montañés-Agudo,Simona Casini,Simona Aufiero,Auriane C Ernault,Ingeborg van der Made,Yigal M Pinto,Carol Ann Remme,Esther E Creemers

Journal of cellular and molecular medicine 25:4583-4595 PubMed33835684

2021

Extracellular vesicle-derived microRNA-18b ameliorates preeclampsia by enhancing trophoblast proliferation and migration via Notch2/TIM3/mTORC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongmei Yang,Nan Shan,Qinyin Deng,Yujue Wang,Yan Hou,Jie Mei,Zhao Wu

Structure (London, England : 1993) 29:320-329.e4 PubMed33275877

2020

Phosphorylation of RIAM by src promotes integrin activation by unmasking the PH domain of RIAM.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ah Cho,Pingfeng Zhang,Vikas Kumar,Mikhail Kavalchuk,Hao Zhang,Qingqiu Huang,James S Duncan,Jinhua Wu

Nature communications 8:1744 PubMed29170462

2017

Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhu,Jun Yang,Thomas Bromberger,Ashley Holly,Fan Lu,Huan Liu,Kevin Sun,Sarah Klapproth,Jamila Hirbawi,Tatiana V Byzova,Edward F Plow,Markus Moser,Jun Qin

Blood 126:2695-703 PubMed26324702

2015

Rap1 and its effector RIAM are required for lymphocyte trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Su,Joseph Wynne,Elaine M Pinheiro,Marianne Strazza,Adam Mor,Emilie Montenont,Jeffrey Berger,David S Paul,Wolfgang Bergmeier,Frank B Gertler,Mark R Philips

The Journal of cell biology 199:317-30 PubMed23045549

2012

Rap1-interacting adapter molecule (RIAM) associates with the plasma membrane via a proximity detector.

Applications

Species

Unspecified reactive species

Joseph P Wynne,Jinhua Wu,Wenjuan Su,Adam Mor,Nikolaos Patsoukis,Vassiliki A Boussiotis,Stevan R Hubbard,Mark R Philips

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RIAM antibody [EPR2806]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] (AB92537)

Western blot - Anti-RIAM antibody [EPR2806] (AB92537)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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