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AB247579

Anti-RIAM antibody [EPR2806] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RIAM antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

PREL1, RARP1, RIAM, APBB1IP, Amyloid beta A4 precursor protein-binding family B member 1-interacting protein, APBB1-interacting protein 1, Proline-rich EVH1 ligand 1, Proline-rich protein 73, Rap1-GTP-interacting adapter molecule, Retinoic acid-responsive proline-rich protein 1, PREL-1, RARP-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] - BSA and Azide free (AB247579)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] - BSA and Azide free (AB247579)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RIAM with ab247579 at 1/2000 (0.449 ug/ml) dilution.

The section was incubated with ab247579 for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] - BSA and Azide free (AB247579)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] - BSA and Azide free (AB247579)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling RIAM with ab247579 at 1/2000 (0.449 ug/ml) dilution.

The section was incubated with ab247579 for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-RIAM antibody [EPR2806] - BSA and Azide free (AB247579)
  • WB

Lab

Western blot - Anti-RIAM antibody [EPR2806] - BSA and Azide free (AB247579)

Exposure time : Lane 1, 10 seconds; Lane 2, 40 seconds

All lanes:

Western blot - Anti-RIAM antibody [EPR2806] - BSA and Azide free (ab247579) at 1/20000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg with 5% NFDM/TBST

Lane 2:

Mouse brain lysate at 15 µg with 5% NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2806

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247579 is the carrier-free version of ab92537.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIAM also known as Rap1-GTP-interacting adaptor molecule is a protein involved in intracellular signaling. RIAM has a molecular weight of approximately 76 kDa. It is expressed in various tissues with a significant presence in hematopoietic cells and endothelial cells. This protein plays a part in connecting cell membrane molecules with actin cytoskeleton therefore promoting cellular adhesion.
Biological function summary

RIAM is essential for the integrin-mediated adhesion processes. It functions as a critical adaptor forming complexes with talin and other signaling proteins which facilitate the activation of integrins. Through these actions RIAM contributes to cell migration immune responses and platelet aggregation. Its interactions influence cellular adhesion and communication pivotal for maintaining tissue structure and response to injuries.

Pathways

RIAM is a vital component in the signaling pathways involving the small GTPase Rap1 which regulates integrins and their effect on cell adhesion. Particularly RIAM serves as a bridge between Rap1 and talin facilitating integrin activation. This process plays an essential role in the focal adhesion pathway where proteins like Kindlin also collaborate to modulate cellular adhesion and movement impacting various physiological processes.

RIAM has connections with immune dysfunction and cardiovascular diseases. For example abnormalities in RIAM-mediated integrin activation can lead to improper immune cell trafficking and related immunodeficiencies. In cardiovascular contexts defective RIAM activity might impair endothelial cell function and contribute to vascular diseases. In these scenarios proteins like talin are closely associated with RIAM often sharing roles in the pathogenesis of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.
See full target information APBB1IP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:666-679 PubMed35170865

2022

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Huang,Ji-Xiong Chen,Yi Ren,Li-Chun Fan,Wei Xiang,Xiao-Jie He
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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