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AB207429

Anti-Ribonuclease 3/ECP antibody [EPR20357]

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(8 Publications)

Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) is a rabbit monoclonal antibody detecting Ribonuclease 3/ECP in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ECP, RNS3, RNASE3, Eosinophil cationic protein, Ribonuclease 3, RNase 3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human Hodgkin's lymphoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human colon [PMID : 11034418].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human spleen [PMID : 11034418].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)
  • WB

Supplier Data

Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 2 seconds; Lane 2 : 3 seconds.

The observed molecular weight is consistent with PMID : 23597768, 19692640.

All lanes:

Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 16-18 kDa

false

Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)
  • WB

Supplier Data

Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) at 1/10000 dilution

Lane 1:

GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160) at 0.01 µg

Lane 2:

GST-His-tagged human Ribonuclease 2 recombinant protein (aa28-160) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 40 kDa

false

Exposure time: 4s

  • Carrier free

    Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20357

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Ribonuclease 3/ECP?
Anti-Ribonuclease 3/ECP [EPR20357] (ab207429) specifically detects a band for Ribonuclease 3/ECP (UniProt: P12724) at a molecular weight of 18kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20357] also available for your convenience: ab207429, Carrier free - ab223148

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease 3 commonly known as Eosinophil Cationic Protein (ECP) is an enzyme with ribonuclease activity. Researchers sometimes refer to it as human RNase 3. The protein has a molecular mass of about 18 to 22 kDa and is highly expressed in eosinophils a type of white blood cell primarily involved in allergic responses and parasitic infections. ECP exhibits cytotoxic bactericidal and antiviral activities contributing to its diverse biological functions.
Biological function summary

Ribonuclease 3 plays a significant role in immune defense mechanisms leveraging its arginine-rich domain to interact with negatively charged microbial surfaces. This interaction allows ECP to participate in the destruction of pathogen cell walls. It is not part of a larger complex but interacts with cellular membranes making it an effective tool against a variety of pathogens. Whether acting through enzymatic activity or by disrupting membrane integrity ECP maintains a pivotal role in host defense.

Pathways

ECP influences processes in both innate and adaptive immune responses. It actively participates in the Th2 cell-mediated immune response which is critical for countering parasitic infections. This protein connects with other components like major basic protein and eosinophil peroxidase through these pathways forming an integrated network that boosts immune responses. Its presence in pathways linked to inflammation and immunity highlights its multifunctionality and importance.

ECP levels correlate with conditions such as asthma and eosinophilic esophagitis. In asthma elevated ECP levels indicate eosinophilic inflammation and airway hyperresponsiveness. Researchers have identified a connection between ECP and proteins like interleukin-5 which modulate eosinophil activity. Similarly in eosinophilic esophagitis increased ECP levels indicate ongoing inflammation and tissue damage providing insights into disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
See full target information RNASE3
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:1058-1068 PubMed40659786

2025

Microbiota-driven antitumour immunity mediated by dendritic cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Yi-Tzu Lin,Shota Fukuoka,Shohei Koyama,Daisuke Motooka,Dieter M Tourlousse,Yuko Shigeno,Yuki Matsumoto,Hiroyuki Yamano,Kazutoshi Murotomi,Hideyuki Tamaki,Takuma Irie,Eri Sugiyama,Shogo Kumagai,Kota Itahashi,Tokiyoshi Tanegashima,Kaori Fujimaki,Sachiko Ito,Mariko Shindo,Takahiro Tsuji,Hiroaki Wake,Keisuke Watanabe,Yuka Maeda,Tomohiro Enokida,Makoto Tahara,Riu Yamashita,Takao Fujisawa,Motoo Nomura,Akihito Kawazoe,Koichi Goto,Toshihiko Doi,Kohei Shitara,Hiroyuki Mano,Yuji Sekiguchi,Shota Nakamura,Yoshimi Benno,Hiroyoshi Nishikawa

Signal transduction and targeted therapy 9:98 PubMed38609366

2024

Infection with SARS-CoV-2 can cause pancreatic impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Deng,Linlin Bao,Zhiqi Song,Ling Zhang,Pin Yu,Yanfeng Xu,Jue Wang,Wenjie Zhao,Xiuqin Zhang,Yunlin Han,Yanhong Li,Jiangning Liu,Qi Lv,Xujian Liang,Fengdi Li,Feifei Qi,Ran Deng,Siyuan Wang,Yibai Xiong,Ruiping Xiao,Hongyang Wang,Chuan Qin

International archives of allergy and immunology 185:274-285 PubMed38029733

2023

Comparing Protein and Gene Expression Signature between Nasal Polyps and Nasal Fluids in Chronic Rhinosinusitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Hou,Changhui Chen,Zhengqi Li,Tong Lu,Lin Sun,Yi Wei,Jian Li,Weiping Wen

Respiratory research 24:221 PubMed37700291

2023

Airway inflammatory changes in the lungs of patients with asthma-COPD overlap (ACO): a bronchoscopy endobronchial biopsy study.

Applications

Unspecified application

Species

Unspecified reactive species

Surajit Dey,Wenying Lu,Greg Haug,Collin Chia,Josie Larby,Heinrich C Weber,Archana Vijay Gaikwad,Prem Bhattarai,Affan Mahmood Shahzad,Prabuddha S Pathinayake,Peter A B Wark,Mathew Suji Eapen,Sukhwinder Singh Sohal

Cancers 14: PubMed36428768

2022

Activated Eosinophils Predict Longer Progression-Free Survival under Immune Checkpoint Inhibition in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine L Ammann,Yasmin F Schwietzer,Christian Mess,Julia-Christina Stadler,Glenn Geidel,Julian Kött,Klaus Pantel,Stefan W Schneider,Jochen Utikal,Alexander T Bauer,Christoffer Gebhardt

Journal of inflammation research 14:3969-3983 PubMed34429629

2021

Necroptosis Underlies Neutrophilic Inflammation Associated with the Chronic Rhinosinusitis with Nasal Polyps (CRSwNP).

Applications

Unspecified application

Species

Unspecified reactive species

Yadong Xie,Min Li,Kun Chen,Haoxiang Zhu,Mengyao Tang,Chun Zhou,Yaoming Zheng,Jing Wen,Miaomiao Han,Jia Zhang,Keqing Zhao,Hui Xiao,Huabin Li

Cellular and molecular life sciences : CMLS 78:2963-2985 PubMed33226440

2020

Human RNase3 immune modulation by catalytic-dependent and independent modes in a macrophage-cell line infection model.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Lu,RanLei Wei,Guillem Prats-Ejarque,Maria Goetz,Gang Wang,Marc Torrent,Ester Boix

Rhinology 56:216-226 PubMed29785413

2018

Multivariate analysis of inflammatory endotypes in recurrent nasal polyposis in a Chinese population.

Applications

Unspecified application

Species

Unspecified reactive species

B Wei,F Liu,J Zhang,Y Liu,J Du,S Liu,N Zhang,C Bachert,J Meng
View all publications
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Product promise

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