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AB281533

Anti-Ribonuclease 3/ECP antibody [EPR23342-100] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Ribonuclease 3/ECP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

ECP, RNS3, RNASE3, Eosinophil cationic protein, Ribonuclease 3, RNase 3

1 Images
Sandwich ELISA - Anti-Ribonuclease 3/ECP antibody [EPR23342-100] - BSA and Azide free (Capture) (AB281533)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Ribonuclease 3/ECP antibody [EPR23342-100] - BSA and Azide free (Capture) (AB281533)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab279415).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23342-100

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab281533 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Eosinophil cationic protein. The recommended pair for sandwich ELISA is:
Capture: ab281533, Human Eosinophil cationic protein Capture Antibody (unconjugated)
Detector: ab281557, Human Eosinophil cationic protein Detector Antibody (unconjugated)
The reference range value is 9.375 - 600 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease 3 commonly known as Eosinophil Cationic Protein (ECP) is an enzyme with ribonuclease activity. Researchers sometimes refer to it as human RNase 3. The protein has a molecular mass of about 18 to 22 kDa and is highly expressed in eosinophils a type of white blood cell primarily involved in allergic responses and parasitic infections. ECP exhibits cytotoxic bactericidal and antiviral activities contributing to its diverse biological functions.
Biological function summary

Ribonuclease 3 plays a significant role in immune defense mechanisms leveraging its arginine-rich domain to interact with negatively charged microbial surfaces. This interaction allows ECP to participate in the destruction of pathogen cell walls. It is not part of a larger complex but interacts with cellular membranes making it an effective tool against a variety of pathogens. Whether acting through enzymatic activity or by disrupting membrane integrity ECP maintains a pivotal role in host defense.

Pathways

ECP influences processes in both innate and adaptive immune responses. It actively participates in the Th2 cell-mediated immune response which is critical for countering parasitic infections. This protein connects with other components like major basic protein and eosinophil peroxidase through these pathways forming an integrated network that boosts immune responses. Its presence in pathways linked to inflammation and immunity highlights its multifunctionality and importance.

ECP levels correlate with conditions such as asthma and eosinophilic esophagitis. In asthma elevated ECP levels indicate eosinophilic inflammation and airway hyperresponsiveness. Researchers have identified a connection between ECP and proteins like interleukin-5 which modulate eosinophil activity. Similarly in eosinophilic esophagitis increased ECP levels indicate ongoing inflammation and tissue damage providing insights into disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
See full target information RNASE3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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