AB203658

Anti-Ribonuclease Inhibitor/RAI antibody

Name: Anti-Ribonuclease Inhibitor/RAI antibody (ab203658) | Abcam
Brand: Abcam Limited
SKU: AB203658
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Ribonuclease Inhibitor/RAI antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNH1 aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PRI, RNH, RNH1, Ribonuclease inhibitor, Placental ribonuclease inhibitor, Ribonuclease/angiogenin inhibitor 1, Placental RNase inhibitor, RAI

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling Ribonuclease Inhibitor/RAI with ab203658 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Ribonuclease Inhibitor/RAI with ab203658 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

All lanes:

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (ab203658) at 1/200 dilution

Lane 1:

Mouse intestine lysate

Lane 2:

Mouse brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

Predicted band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RNH1 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P13489

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease Inhibitor (RI) also known as RAI is a protein weighing approximately 50 kDa and is responsible for binding to ribonucleases (RNases) to inhibit their activity. This protein is expressed in a variety of tissues but appears most abundantly in the cytoplasm of mammalian cells. RI is characterized by its leucine-rich repeat (LRR) structure which facilitates its high-affinity binding to target RNases effectively serving to regulate ribonuclease activity within cells.

Biological function summary

Ribonuclease Inhibitor participates in protecting cellular RNA from degradation by forming stable complexes with RNases. This interaction is important for maintaining RNA stability particularly within cellular environments where mRNA turnover must be tightly controlled. RI does not only inhibit free RNases but also regulates those RNases that are secreted or released subsequently. It does not have enzymatic activity itself serving more as a guard that ensures RNA integrity for processes such as transcription translation and overall gene expression.

Pathways

The function of Ribonuclease Inhibitor fits strategically into RNA metabolism and the regulation of its turnover under various physiological conditions. RI acts within the mRNA biogenesis and degradation pathways coordinating with other proteins involved in RNA processing such as Dicer. RI's role is essential in fine-tuning RNA levels and preventing unwanted cellulalr RNA cleavage which can be directed by pathways involving diverse sets of RNases across different cellular contexts.

Researchers have observed connections between Ribonuclease Inhibitor and conditions such as cancer and neurodegenerative diseases. For instance disrupting RI balance can result in excessive RNA degradation contributing to pathologies associated with abnormal mRNA destruction in cells. It often aligns in regulatory functions with proteins like angiogenin where disrupted RI-angiogenin interactions have been implicated in tumor growth and progression. Additionally RI's dysfunction is associated with neuroinflammatory conditions where RNA stability plays critical roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed : 12578357, PubMed : 14515218, PubMed : 3219362, PubMed : 3243277, PubMed : 3470787, PubMed : 9050852). May play a role in redox homeostasis (PubMed : 17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed : 23843625, PubMed : 32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed : 23843625).

See full target information RNH1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38203210

2024

Insights into the Cellular Localization and Functional Properties of TSPYL5 Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey A Silonov,Eugene Y Smirnov,Eva A Shmidt,Irina M Kuznetsova,Konstantin K Turoverov,Alexander V Fonin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ribonuclease Inhibitor/RAI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (AB203658)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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