AB245627

Anti-Ribonuclease Inhibitor/RAI antibody

Name: Anti-Ribonuclease Inhibitor/RAI antibody (ab245627) | Abcam
Brand: Abcam Limited
SKU: AB245627
Price: 525 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(1 Publication)

Rabbit Polyclonal Ribonuclease Inhibitor/RAI antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNH1 aa 1-50.

View Alternative Names

PRI, RNH, RNH1, Ribonuclease inhibitor, Placental ribonuclease inhibitor, Ribonuclease/angiogenin inhibitor 1, Placental RNase inhibitor, RAI

2 Images

Supplier Data

Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (AB245627)

Ribonuclease Inhibitor/RAI was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245627 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab245627 at 0.4 μg/ml.

Lane 1 : ab245627 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (ab245627)

Predicted band size: 50 kDa

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Supplier Data

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (AB245627)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (ab245627) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RNH1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P13489

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245627 was affinity purified using an epitope specific to Ribonuclease Inhibitor/RAI immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease Inhibitor (RI) also known as RAI is a protein weighing approximately 50 kDa and is responsible for binding to ribonucleases (RNases) to inhibit their activity. This protein is expressed in a variety of tissues but appears most abundantly in the cytoplasm of mammalian cells. RI is characterized by its leucine-rich repeat (LRR) structure which facilitates its high-affinity binding to target RNases effectively serving to regulate ribonuclease activity within cells.

Biological function summary

Ribonuclease Inhibitor participates in protecting cellular RNA from degradation by forming stable complexes with RNases. This interaction is important for maintaining RNA stability particularly within cellular environments where mRNA turnover must be tightly controlled. RI does not only inhibit free RNases but also regulates those RNases that are secreted or released subsequently. It does not have enzymatic activity itself serving more as a guard that ensures RNA integrity for processes such as transcription translation and overall gene expression.

Pathways

The function of Ribonuclease Inhibitor fits strategically into RNA metabolism and the regulation of its turnover under various physiological conditions. RI acts within the mRNA biogenesis and degradation pathways coordinating with other proteins involved in RNA processing such as Dicer. RI's role is essential in fine-tuning RNA levels and preventing unwanted cellulalr RNA cleavage which can be directed by pathways involving diverse sets of RNases across different cellular contexts.

Researchers have observed connections between Ribonuclease Inhibitor and conditions such as cancer and neurodegenerative diseases. For instance disrupting RI balance can result in excessive RNA degradation contributing to pathologies associated with abnormal mRNA destruction in cells. It often aligns in regulatory functions with proteins like angiogenin where disrupted RI-angiogenin interactions have been implicated in tumor growth and progression. Additionally RI's dysfunction is associated with neuroinflammatory conditions where RNA stability plays critical roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed : 12578357, PubMed : 14515218, PubMed : 3219362, PubMed : 3243277, PubMed : 3470787, PubMed : 9050852). May play a role in redox homeostasis (PubMed : 17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed : 23843625, PubMed : 32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed : 23843625).

See full target information RNH1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 19:3519-3526 PubMed30896869

2019

Overexpression of ribonuclease inhibitor induces autophagy in human colorectal cancer cells via the Akt/mTOR/ULK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Tang,Feng Ren,Xi Cong,Ying Kong,Yuxiang Tian,Yuefei Xu,Jianhui Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ribonuclease Inhibitor/RAI antibody

Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (AB245627)

Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (AB245627)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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