AB157101

Anti-Ribosomal protein S11/RPS11 antibody

Name: Anti-Ribosomal protein S11/RPS11 antibody (ab157101) | Abcam
Brand: Abcam Limited
SKU: AB157101
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Ribosomal protein S11/RPS11 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human 40S ribosomal protein S11 aa 1-50.

View Alternative Names

Small ribosomal subunit protein uS17, 40S ribosomal protein S11, RPS11

2 Images

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Immunoprecipitation - Anti-Ribosomal protein S11/RPS11 antibody (AB157101)

Detection of Ribosomal protein S11/RPS11 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157101 at 6 μg/mg lysate for IP (Lane 1). For WB detection an ab157101 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Ribosomal protein S11/RPS11 antibody (ab157101)

Predicted band size: 18 kDa

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Unknown

Western blot - Anti-Ribosomal protein S11/RPS11 antibody (AB157101)

All lanes:

Western blot - Anti-Ribosomal protein S11/RPS11 antibody (ab157101) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 18 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human 40S ribosomal protein S11 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P62280

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab157101 was affinity purified using an epitope specific to Ribosomal protein S11/RPS11 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribosomal protein S11 also known as RPS11 or BSA S11 is a component of the small 40S subunit of ribosomes. It has a mass of about 18.8 kDa. RPS11 is expressed across various tissues essential for protein synthesis in eukaryotic cells. It plays a mechanical role in facilitating the assembly and proper function of the ribosome which is critical for translating mRNA into protein.

Biological function summary

RPS11 serves as an important player in the ribosomal complex vital for maintaining the structure and function of the ribosome. It assists in the binding of mRNA and tRNA promoting the translation process. The precise arrangement of RPS11 and its interactions with other ribosomal proteins ensures efficient protein synthesis necessary for cell growth and function.

Pathways

RPS11 plays a significant role in the translation mechanism including the mTOR pathway which regulates cell growth in response to nutrients and growth factors. Through this pathway RPS11 works in conjunction with proteins like S6 kinase and 4E-BP1 modulating protein translation rates in various cellular conditions. Additionally RPS11 contributes to the ribosomal biogenesis pathway integrating signals for assembly and function of ribosomes.

Disruptions in RPS11 regulation or mutations can lead to conditions such as Diamond-Blackfan anemia characterized by reduced red blood cell production. RPS11's interaction with other ribosomal proteins like RPS19 has been implicated in this disorder. Moreover altered expression of RPS11 may play a role in various cancers influencing tumor growth and malignancy due to abnormal protein synthesis rates.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information 40S ribosomal protein S11

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 188:5384-5402.e25 PubMed40701149

2025

SPIDR enables multiplexed mapping of RNA-protein interactions and uncovers a mechanism for selective translational suppression upon cell stress.

Applications

Unspecified application

Species

Unspecified reactive species

Erica Wolin,Jimmy K Guo,Mario R Blanco,Isabel N Goronzy,Darvesh Gorhe,Wenzhao Dong,Andrew A Perez,Abdurrahman Keskin,Elizabeth Valenzuela,Ahmed A Abdou,Carl R Urbinati,Ross Kaufhold,H Tomas Rube,Jailson Brito Querido,Mitchell Guttman,Marko Jovanovic

Science (New York, N.Y.) 386:eadq8587 PubMed39571015

2024

Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Zhu,Victor Emmanuel Cruz,He Zhang,Jan P Erzberger,Joshua T Mendell

PLoS biology 22:e3002767 PubMed39316623

2024

A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist.

Applications

Unspecified application

Species

Unspecified reactive species

Jérémie Le Pen,Gabrielle Paniccia,Volker Kinast,Marcela Moncada-Velez,Alison W Ashbrook,Michael Bauer,H-Heinrich Hoffmann,Ana Pinharanda,Inna Ricardo-Lax,Ansgar F Stenzel,Edwin A Rosado-Olivieri,Kenneth H Dinnon,William C Doyle,Catherine A Freije,Seon-Hui Hong,Danyel Lee,Tyler Lewy,Joseph M Luna,Avery Peace,Carltin Schmidt,William M Schneider,Roni Winkler,Elaine Z Yip,Chloe Larson,Timothy McGinn,Miriam-Rose Menezes,Lavoisier Ramos-Espiritu,Priyam Banerjee,John T Poirier,Francisco J Sànchez-Rivera,Aurélie Cobat,Qian Zhang,Jean-Laurent Casanova,Thomas S Carroll,J Fraser Glickman,Eleftherios Michailidis,Brandon Razooky,Margaret R MacDonald,Charles M Rice

Current oncology (Toronto, Ont.) 28:2150-2172 PubMed34208001

2021

Dual Targeting of Sorafenib-Resistant HCC-Derived Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Shrestha,Kim R Bridle,Lu Cao,Darrell H G Crawford,Aparna Jayachandran

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ribosomal protein S11/RPS11 antibody

Immunoprecipitation - Anti-Ribosomal protein S11/RPS11 antibody (AB157101)

Western blot - Anti-Ribosomal protein S11/RPS11 antibody (AB157101)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise