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AB97808

Anti-RIC8A antibody

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(3 Publications)

Rabbit Polyclonal RIC8A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RIC8A aa 500 to C-terminus.

View Alternative Names

Chaperone Ric-8A, Synembryn-A, RIC8A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (AB97808)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (AB97808)

ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.

Western blot - Anti-RIC8A antibody (AB97808)
  • WB

Unknown

Western blot - Anti-RIC8A antibody (AB97808)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RIC8A antibody (ab97808) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RIC8A aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NPQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIC8A also known as Synembryn-A is a guanine nucleotide exchange factor that plays an important mechanical role in cellular signaling. It catalyzes the exchange of GDP for GTP on G protein alpha subunits activating them. RIC8A weighs approximately 60 kDa and is mainly cytoplasmic. Researchers have identified its expression in several tissues such as the brain heart and lungs indicating a wide pattern of activity in the body.
Biological function summary

RIC8A is involved in intracellular signaling and regulation of G-protein coupled receptor (GPCR) pathways. It does not directly form part of a stable complex but interacts transiently with heterotrimeric G proteins during activation. Through these interactions RIC8A influences processes such as neurotransmitter release cell division and hormone secretion. It also affects synaptic signals and is important for proper brain function.

Pathways

RIC8A plays a significant role in the Wnt signaling and synaptic transmission pathways. It operates by interacting with GPCR-related proteins like G-alpha subunits which are coupled later to downstream effectors. In the Wnt signaling pathway RIC8A supports signal propagation critical for cell fate determination. The synaptic transmission pathway further benefits from RIC8A's role in modulating neurotransmitter release making it essential for neural communication.

Researchers have linked RIC8A dysfunction to various neurological disorders and cancer. Abnormal RIC8A function can contribute to conditions like epilepsy and certain types of tumor growth owing to its role in cell signaling and division. The interaction of RIC8A with proteins like G-alpha subunits and components of the Wnt signaling pathway highlights its involvement in these diseases. Such associations suggest potential therapeutic target opportunities for addressing these complex conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity : folds GNAI1, GNAO1, GNA13 and GNAQ (By similarity). Does not fold G(s) G-alpha proteins GNAS nor GNAL (By similarity). Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP (By similarity). Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein (GNAI1), possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (PubMed : 16629901).
See full target information RIC8A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 8: PubMed31741433

2019

Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zeng,Johnny Castillo-Cabrera,Mika Manser,Bo Lu,Zinger Yang,Vaik Strande,Damien Begue,Raffaella Zamponi,Shumei Qiu,Frederic Sigoillot,Qiong Wang,Alicia Lindeman,John S Reece-Hoyes,Carsten Russ,Debora Bonenfant,Xiaomo Jiang,Youzhen Wang,Feng Cong

Genes to cells : devoted to molecular & cellular mechanisms 22:293-309 PubMed28185378

2017

Ric-8A, an activator protein of Gαi, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kanako Chishiki,Sachiko Kamakura,Junya Hayase,Hideki Sumimoto

PloS one 9:e86680 PubMed24466196

2014

Resistance to inhibitors of cholinesterase (Ric)-8A and Gαi contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity.

Applications

IP, WB, ICC/IF

Species

Human, Human, Human

Cedric Boularan,Olena Kamenyeva,Hyeseon Cho,John H Kehrl
View all publications
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