Rabbit Polyclonal RIC8A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RIC8A aa 500 to C-terminus.
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Publications
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- eLife 8:2019Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zeng et. al.PubMed 31741433
- Genes to cells : devoted to molecular & cellular mechanisms 22:293-3092017Ric-8A, an activator protein of Gαi, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanako Chishiki,Sachiko Kamakura,Junya Hayase,Hideki SumimotoPubMed 28185378
- PloS one 9:e866802014Resistance to inhibitors of cholinesterase (Ric)-8A and Gαi contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RIC8A aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPQ8
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity: folds GNAI1, GNAO1, GNA13 and GNAQ (By similarity). Does not fold G(s) G-alpha proteins GNAS nor GNAL (By similarity). Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP (By similarity). Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein (GNAI1), possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (PubMed:16629901).
Alternative names
Chaperone Ric-8A, Synembryn-A, RIC8A
Recommended products
Rabbit Polyclonal RIC8A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RIC8A aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RIC8A aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPQ8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIC8A also known as Synembryn-A is a guanine nucleotide exchange factor that plays an important mechanical role in cellular signaling. It catalyzes the exchange of GDP for GTP on G protein alpha subunits activating them. RIC8A weighs approximately 60 kDa and is mainly cytoplasmic. Researchers have identified its expression in several tissues such as the brain heart and lungs indicating a wide pattern of activity in the body.
- Biological function summary
RIC8A is involved in intracellular signaling and regulation of G-protein coupled receptor (GPCR) pathways. It does not directly form part of a stable complex but interacts transiently with heterotrimeric G proteins during activation. Through these interactions RIC8A influences processes such as neurotransmitter release cell division and hormone secretion. It also affects synaptic signals and is important for proper brain function.
- Pathways
RIC8A plays a significant role in the Wnt signaling and synaptic transmission pathways. It operates by interacting with GPCR-related proteins like G-alpha subunits which are coupled later to downstream effectors. In the Wnt signaling pathway RIC8A supports signal propagation critical for cell fate determination. The synaptic transmission pathway further benefits from RIC8A's role in modulating neurotransmitter release making it essential for neural communication.
- Associated diseases and disorders
Researchers have linked RIC8A dysfunction to various neurological disorders and cancer. Abnormal RIC8A function can contribute to conditions like epilepsy and certain types of tumor growth owing to its role in cell signaling and division. The interaction of RIC8A with proteins like G-alpha subunits and components of the Wnt signaling pathway highlights its involvement in these diseases. Such associations suggest potential therapeutic target opportunities for addressing these complex conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-RIC8A antibody (ab97808)
7.5% SDS PAGE
All lanes: Western blot - Anti-RIC8A antibody (ab97808) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: HeLa whole cell lysate at 30 µg
Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (ab97808)
ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.
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