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AB170006

Anti-RIC8B antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal RIC8B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Synembryn-B aa 150-300.

View Alternative Names

Chaperone Ric-8B, Brain synembryn, Synembryn-B, hSyn, RIC8B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8B antibody (AB170006)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8B antibody (AB170006)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal brain tissue, labeling RIC8B with ab170006 at 1/100 dilution.

Western blot - Anti-RIC8B antibody (AB170006)
  • WB

Supplier Data

Western blot - Anti-RIC8B antibody (AB170006)

All lanes:

Western blot - Anti-RIC8B antibody (ab170006) at 1/1000 dilution

Lane 1:

Human fetal brain lysate

Lane 2:

HeLa cell lysate

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Synembryn-B aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q9NVN3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIC8B also known as Resistance to Inhibitors of Cholinesterase 8 homolog B is a guanine nucleotide exchange factor that interacts with G protein alpha subunits. It has a molecular weight of approximately 60 kDa. This protein is expressed in many tissues with high levels reported in areas of the brain such as the cerebral cortex and cerebellum. RIC8B plays an important role in modulating signal transduction by facilitating the replacement of bound GDP with GTP on G protein alpha subunits which is an essential step in signal transducing processes.
Biological function summary

RIC8B acts as a regulatory element in G protein signaling. It is not a permanent part of a protein complex but associates transiently with G alpha subunits to promote their active form. These interactions are critical for downstream signaling cascades that influence cellular responses to external stimuli. RIC8B's role in conjunction with G protein-coupled receptors (GPCRs) affects how cells perceive and respond to a wide range of signals including hormones neurotransmitters and other signaling molecules.

Pathways

RIC8B influences multiple signaling pathways notably the GPCR and olfactory signaling pathways. In GPCR signaling RIC8B interacts with G protein alpha subunits such as GNAI and GNAS to enhance the signal transduction process. Through these pathways RIC8B affects various physiological processes like olfaction and balance. Additionally its regulation of G alpha proteins integrates with other proteins such as GPCR kinases to modulate receptor desensitization and signaling duration.

RIC8B has connections to neurodegenerative disorders like Alzheimer's and bipolar disorder. Disruption of its function can impair neurotransmitter signaling thereby influencing disease pathways. RIC8B's interactions with proteins like ApoE in Alzheimer's and with neurotransmitter systems in mood disorders suggest roles in disease mechanisms. This underlines the importance of RIC8B as a target for understanding neuropathological conditions and developing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chaperone that specifically binds and folds nascent G(s) G-alpha proteins (GNAS and GNAL) prior to G protein heterotrimer formation, promoting their association with the plasma membrane (By similarity). Also acts as a guanine nucleotide exchange factor (GEF) for G(s) proteins by stimulating exchange of bound GDP for free GTP (By similarity). Acts as an important component for odorant signal transduction by mediating GNAL (G(olf)-alpha) folding, thereby promoting-dependent cAMP accumulation in olfactory sensory neurons (By similarity).
See full target information Synembryn-B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107470 PubMed38879012

2024

The ric-8b protein (resistance to inhibitors of cholinesterase 8b) is key to preserving contractile function in the adult heart.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Tsisanova,Muriel Nobles,Sonia Sebastian,Keat-Eng Ng,Alison Thomas,Lee Scott Weinstein,Patricia B Munroe,Andrew Tinker

Cell host & microbe 16:31-42 PubMed25011106

2014

Measles virus suppresses RIG-I-like receptor activation in dendritic cells via DC-SIGN-mediated inhibition of PP1 phosphatases.

Applications

Unspecified application

Species

Unspecified reactive species

Annelies W Mesman,Esther M Zijlstra-Willems,Tanja M Kaptein,Rik L de Swart,Meredith E Davis,Martin Ludlow,W Paul Duprex,Michaela U Gack,Sonja I Gringhuis,Teunis B H Geijtenbeek
View all publications
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Product promise

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