AB104838

Anti-RICTOR antibody [7B3]

Name: Anti-RICTOR antibody [7B3] (ab104838) | Abcam
Brand: Abcam Limited
SKU: AB104838
Price: 510 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal RICTOR antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RICTOR.

View Alternative Names

KIAA1999, RICTOR, Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3

5 Images

Flow Cytometry - Anti-RICTOR antibody [7B3] (AB104838)

Flow Cyt

Unknown

Flow Cytometry - Anti-RICTOR antibody [7B3] (AB104838)

ab104838 used for Flow cytometric analysis of HeLa cells (green). The negative control is shown in purple.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [7B3] (AB104838)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [7B3] (AB104838)

ab104838 at 1/200 dilution staining RICTOR in paraffin-fixed Human breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Immunocytochemistry/ Immunofluorescence - Anti-RICTOR antibody [7B3] (AB104838)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RICTOR antibody [7B3] (AB104838)

ab104838 at 1/200 dilution staining RICTOR in NIH3T3 cells (green) by immunofluorescence. DRAQ5 fluorescent DNA dye is shown in blue. Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [7B3] (AB104838)

ab104838 at 1/200 dilution staining RICTOR in paraffin-embedded thyroid gland tissues by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

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Western blot - Anti-RICTOR antibody [7B3] (AB104838)

All lanes:

Western blot - Anti-RICTOR antibody [7B3] (ab104838) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

PANC-1 cell lysate

Lane 3:

MOLT4 cell lysate

Lane 4:

HepG2 cell lysate

Lane 5:

HEK293 cell lysate

Predicted band size: 192 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7B3

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RICTOR. The exact immunogen used to generate this antibody is proprietary information.

Q6R327

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR173148-16 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RICTOR (Rapamycin-insensitive companion of mTOR) is an important component of the mTOR complex 2 (mTORC2) distinguished by its ability to interact with mTOR (mechanistic target of rapamycin). RICTOR possesses a molecular mass of approximately 192 kDa. You can find RICTOR widely expressed in various tissues including the brain liver and skeletal muscle. Researchers also refer to it as AVO3.

Biological function summary

The protein plays a significant role in cellular growth proliferation and survival. RICTOR forms part of the mTORC2 complex which is important for Akt/PKB phosphorylation. Activation of mTORC2 by RICTOR regulates actin cytoskeleton dynamics and membrane trafficking. This involvement affects the control of cell metabolism and immune responses.

Pathways

RICTOR integrates into the PI3K/Akt signaling pathway influencing cell growth and survival in response to nutrients and growth factors. RICTOR as part of mTORC2 also interacts with proteins such as Sin1 and mLST8 helping regulate PKB/Akt phosphorylation at Ser473. This interaction links RICTOR's function to the insulin signaling pathway which controls aspects of glucose metabolism.

The dysregulation of RICTOR plays a role in cancer progression and metabolic disorders. Alterations in the PI3K/Akt/mTOR signaling pathway can lead to the development of various cancers with RICTOR contributing to tumorigenesis through Akt activation. Additionally aberrant RICTOR expression affects insulin signaling therefore connecting it to diabetes. Through these pathways and conditions RICTOR's interaction with proteins such as mTOR illustrates its relevance in disease mechanisms and therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed : 15268862, PubMed : 15718470, PubMed : 19720745, PubMed : 19995915, PubMed : 21343617, PubMed : 33158864, PubMed : 35904232, PubMed : 35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed : 19720745, PubMed : 19935711, PubMed : 19995915). In contrast to mTORC1, mTORC2 is nutrient-insensitive (PubMed : 15467718, PubMed : 21343617). Within the mTORC2 complex, RICTOR probably acts as a molecular adapter (PubMed : 21343617, PubMed : 33158864, PubMed : 35926713). RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2 (PubMed : 33158864). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed : 15718470, PubMed : 19720745, PubMed : 19935711, PubMed : 35926713). mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (By similarity). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed : 15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed : 15467718).

See full target information RICTOR

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:4226-4251 PubMed39026009

2024

Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Misha Kalarikkal,Rimpi Saikia,Lizanne Oliveira,Yashashree Bhorkar,Akshay Lonare,Pallavi Varshney,Prathamesh Dhamale,Amitabha Majumdar,Jomon Joseph

Molecular medicine reports 22:5293-5303 PubMed33174028

2020

S100A8 and S100A9 promote endothelial cell activation through the RAGE‑mediated mammalian target of rapamycin complex 2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhong,Fengwen Xie,Li Chen,Zhixing Liu,Qun Wang

Neoplasma 67:501-508 PubMed32039630

2020

Expression of Raptor and Rictor and their relationships with angiogenesis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

F F Wen,X Y Li,Y Y Li,S He,X Y Xu,Y H Liu,L Liu,S H Wu

Neuropsychopharmacology : official publication of 45:1059-1067 PubMed31952070

2020

Downregulated AKT-mTOR signaling pathway proteins in dorsolateral prefrontal cortex in Schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Radhika Chadha,James H Meador-Woodruff

Apoptosis : an international journal on programmed cell death 24:369-381 PubMed30756206

2019

Minocycline promotes cardiomyocyte mitochondrial autophagy and cardiomyocyte autophagy to prevent sepsis-induced cardiac dysfunction by Akt/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Erfei Zhang,Xiaoying Zhao,Li Zhang,Nan Li,Jinqi Yan,Ke Tu,Ruhu Yan,Jianqiang Hu,Mingming Zhang,Dongdong Sun,Lichao Hou

Nature communications 9:257 PubMed29343687

2018

Mechanically-sensitive miRNAs bias human mesenchymal stem cell fate via mTOR signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica E Frith,Gina D Kusuma,James Carthew,Fanyi Li,Nicole Cloonan,Guillermo A Gomez,Justin J Cooper-White

Neuro-oncology 15:1604-14 PubMed24203892

2013

Activation of mTORC1/mTORC2 signaling in pediatric low-grade glioma and pilocytic astrocytoma reveals mTOR as a therapeutic target.

Applications

IHC-P

Species

Human

Marianne Hütt-Cabezas,Matthias A Karajannis,David Zagzag,Smit Shah,Iren Horkayne-Szakaly,Elisabeth J Rushing,J Douglas Cameron,Deepali Jain,Charles G Eberhart,Eric H Raabe,Fausto J Rodriguez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RICTOR antibody [7B3]

Flow Cytometry - Anti-RICTOR antibody [7B3] (AB104838)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [7B3] (AB104838)

Immunocytochemistry/ Immunofluorescence - Anti-RICTOR antibody [7B3] (AB104838)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody [7B3] (AB104838)

Western blot - Anti-RICTOR antibody [7B3] (AB104838)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise