AB70374

Anti-RICTOR antibody

Name: Anti-RICTOR antibody (ab70374) | Abcam
Brand: Abcam Limited
SKU: AB70374

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(20 Publications)

Rabbit Polyclonal RICTOR antibody. Suitable for IHC-P, IP, Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human RICTOR.

View Alternative Names

KIAA1999, RICTOR, Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (AB70374)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (AB70374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling RICTOR with ab70374 at 1/1000 (1µg/ml). Detection : DAB.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (AB70374)

ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Unknown

Immunoprecipitation - Anti-RICTOR antibody (AB70374)

Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG.

All lanes:

Immunoprecipitation - Anti-RICTOR antibody (ab70374)

Predicted band size: 192 kDa

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Unknown

Western blot - Anti-RICTOR antibody (AB70374)

All lanes:

Western blot - Anti-RICTOR antibody (ab70374) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 5 µg

Lane 2:

HeLa whole cell lysate at 2.5 µg

Lane 3:

HeLa whole cell lysate at 1 µg

Lane 4:

HeLa whole cell lysate at 0.5 µg

Lane 5:

NIH3T3 cells at 50 µg

Predicted band size: 192 kDa

Observed band size: 192 kDa,78 kDa,80 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, Flow Cyt, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RICTOR. The exact immunogen used to generate this antibody is proprietary information.

Q6R327

Specificity

Initial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "0.5 ug per 1 million cells in a 150 mcl reaction. <a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "0.5 ug per 1 million cells in a 150 mcl reaction. <a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RICTOR (Rapamycin-insensitive companion of mTOR) is an important component of the mTOR complex 2 (mTORC2) distinguished by its ability to interact with mTOR (mechanistic target of rapamycin). RICTOR possesses a molecular mass of approximately 192 kDa. You can find RICTOR widely expressed in various tissues including the brain liver and skeletal muscle. Researchers also refer to it as AVO3.

Biological function summary

The protein plays a significant role in cellular growth proliferation and survival. RICTOR forms part of the mTORC2 complex which is important for Akt/PKB phosphorylation. Activation of mTORC2 by RICTOR regulates actin cytoskeleton dynamics and membrane trafficking. This involvement affects the control of cell metabolism and immune responses.

Pathways

RICTOR integrates into the PI3K/Akt signaling pathway influencing cell growth and survival in response to nutrients and growth factors. RICTOR as part of mTORC2 also interacts with proteins such as Sin1 and mLST8 helping regulate PKB/Akt phosphorylation at Ser473. This interaction links RICTOR's function to the insulin signaling pathway which controls aspects of glucose metabolism.

The dysregulation of RICTOR plays a role in cancer progression and metabolic disorders. Alterations in the PI3K/Akt/mTOR signaling pathway can lead to the development of various cancers with RICTOR contributing to tumorigenesis through Akt activation. Additionally aberrant RICTOR expression affects insulin signaling therefore connecting it to diabetes. Through these pathways and conditions RICTOR's interaction with proteins such as mTOR illustrates its relevance in disease mechanisms and therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output (PubMed : 15268862, PubMed : 15718470, PubMed : 19720745, PubMed : 19995915, PubMed : 21343617, PubMed : 33158864, PubMed : 35904232, PubMed : 35926713). In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1 (PubMed : 19720745, PubMed : 19935711, PubMed : 19995915). In contrast to mTORC1, mTORC2 is nutrient-insensitive (PubMed : 15467718, PubMed : 21343617). Within the mTORC2 complex, RICTOR probably acts as a molecular adapter (PubMed : 21343617, PubMed : 33158864, PubMed : 35926713). RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2 (PubMed : 33158864). mTORC2 plays a critical role in AKT1 activation by mediating phosphorylation of different sites depending on the context, such as 'Thr-450', 'Ser-473', 'Ser-477' or 'Thr-479', facilitating the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDPK1/PDK1 which is a prerequisite for full activation (PubMed : 15718470, PubMed : 19720745, PubMed : 19935711, PubMed : 35926713). mTORC2 catalyzes the phosphorylation of SGK1 at 'Ser-422' and of PRKCA on 'Ser-657' (By similarity). The mTORC2 complex also phosphorylates various proteins involved in insulin signaling, such as FBXW8 and IGF2BP1 (By similarity). mTORC2 acts upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed : 15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed : 15467718).

See full target information RICTOR

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401140 PubMed39517124

2024

Thermogenic Adipocytes Promote M2 Macrophage Polarization through CNNM4-Mediated Mg Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Anke Zhang,Junkun Jiang,Chuan Zhang,Houshi Xu,Wenjing Yu,Zhen-Ning Zhang,Ling Yuan,Zhangming Lu,Yuqing Deng,Haonan Fan,Chaoyou Fang,Xiaoyu Wang,Anwen Shao,Sheng Chen,Huaming Li,Jiahua Ni,Wenhui Wang,Xiaonong Zhang,Jianmin Zhang,Bing Luan

eLife 13: PubMed39283662

2024

Mechanistic target of rapamycin (mTOR) pathway in Sertoli cells regulates age-dependent changes in sperm DNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Saira Amir,Olatunbosun Arowolo,Ekaterina Mironova,Joseph McGaunn,Oladele Oluwayiose,Oleg Sergeyev,J Richard Pilsner,Alexander Suvorov

Autophagy 20:659-674 PubMed38290972

2024

ANXA2 (annexin A2) is crucial to ATG7-mediated autophagy, leading to tumor aggressiveness in triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minsoo Koh,Hyesol Lim,Hao Jin,Minjoo Kim,Yeji Hong,Young Keun Hwang,Yunjung Woo,Eun-Sook Kim,Sun Young Kim,Kyung Mee Kim,Hyun Kyung Lim,Joohee Jung,Sujin Kang,Boyoun Park,Han-Byoel Lee,Wonshik Han,Myung-Shik Lee,Aree Moon

International journal of molecular sciences 23: PubMed35955931

2022

PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2.

Applications

Unspecified application

Species

Unspecified reactive species

Qiongfeng Chen,Jingguang Jin,Wenhui Guo,Zhimin Tang,Yunfei Luo,Ying Ying,Hui Lin,Zhijun Luo

International journal of biological sciences 18:4869-4883 PubMed35982899

2022

Epithelial-mesenchymal reprogramming by KLF4-regulated Rictor expression contributes to metastasis of non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huiling Zhou,Qing Guan,Xuyang Hou,Lijun Liu,Li Zhou,Wei Li,Haidan Liu

Nature communications 13:3301 PubMed35676256

2022

Targeting parvalbumin promotes M2 macrophage polarization and energy expenditure in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojian Lin,Anke Zhang,Ling Yuan,Yufan Wang,Chuan Zhang,Junkun Jiang,Houshi Xu,Huiwen Yuan,Hui Yao,Qianying Zhang,Yong Zhang,Meiqing Lou,Ping Wang,Zhen-Ning Zhang,Bing Luan

Frontiers in oncology 11:641453 PubMed34540654

2021

Rictor Activates Cav 1 Through the Akt Signaling Pathway to Inhibit the Apoptosis of Gastric Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Zhen Cao,Li Min,Si Liu,Ru-Yue Tian,Hai-Yan Jiang,Juan Liu,Lin-Lin Shao,Rui Cheng,Sheng-Tao Zhu,Shui-Long Guo,Peng Li

Cell reports 33:108564 PubMed33378666

2020

mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of RICTOR.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Wrobel,Farah H Siddiqi,Sandra M Hill,Sung Min Son,Cansu Karabiyik,Hyunjeong Kim,David C Rubinsztein

Molecular cancer research : MCR 18:1863-1875 PubMed32873626

2020

G-Protein-Coupled Estrogen Receptor 1 Promotes Gender Disparities in Hepatocellular Carcinoma via Modulation of SIN1 and mTOR Complex 2 Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Guanying Feng,Jingshu Cai,Yunchuanxiang Huang,Xianjun Zhu,Bo Gong,Zhenglin Yang,Chunhong Yan,Zhuowei Hu,Lu Yang,Ziyan Wang

Cell death discovery 6:40 PubMed32528729

2020

Loss of Rictor in tubular cells exaggerates lipopolysaccharide induced renal inflammation and acute kidney injury via Yap/Taz-NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gui,Qing Hou,Qingmiao Lu,Chunsun Dai,Jianzhong Li

View all publications

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Anti-RICTOR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (AB70374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (AB70374)

Immunoprecipitation - Anti-RICTOR antibody (AB70374)

Western blot - Anti-RICTOR antibody (AB70374)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise