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AB224447

Anti-RIDA antibody

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(1 Publication)

Rabbit Polyclonal RIDA antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RIDA aa 50 to C-terminus.

View Alternative Names

HRSP12, RIDA, 2-iminobutanoate/2-iminopropanoate deaminase, 14.5 kDa translational inhibitor protein, Heat-responsive protein 12, Reactive intermediate imine deaminase A homolog, Translation inhibitor L-PSP ribonuclease, UK114 antigen homolog, hp14.5, p14.5

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human kidney stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human liver stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human Fallopian tube stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human skin stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

Western blot - Anti-RIDA antibody (AB224447)
  • WB

Supplier Data

Western blot - Anti-RIDA antibody (AB224447)

All lanes:

Western blot - Anti-RIDA antibody (ab224447) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver lysate

Predicted band size: 14 kDa

false

Western blot - Anti-RIDA antibody (AB224447)
  • WB

Supplier Data

Western blot - Anti-RIDA antibody (AB224447)

All lanes:

Western blot - Anti-RIDA antibody (ab224447) at 1/100 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Rat liver lysate

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RIDA aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52758

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIDA also known as Reactive Intermediate Deaminase A functions as an important enzyme in cellular processes. This protein has a molecular weight of approximately 19 kDa and is prominently expressed in various tissues including the liver and kidneys. RIDA acts by deaminating reactive intermediates specifically 2-aminoacrylate which in turn prevents toxic accumulation within cells. Its expression sees influence under various metabolic stress conditions indicating its important role in maintaining cellular homeostasis.
Biological function summary

Reactive intermediate deaminase A supports metabolic resilience by engaging in the nitrogen metabolism pathway. It functions as part of a deaminase complex important for processing amino acid intermediates. During this metabolic function RIDA catalyzes the conversion of potentially toxic compounds into less harmful ones facilitating their subsequent expulsion or further processing. Such activity helps maintain the balance of available nitrogenous compounds in the cell which is necessary for proper cellular function.

Pathways

Reactive intermediate deaminase A integrates into the amino acid metabolism and the central nitrogen metabolism pathways. These pathways involve several interactive proteins like serine hydroxymethyltransferase supporting the correct regulation and maintenance of cellular nitrogen balance. RIDA collaborates with these proteins to ensure that amino acid degradation is smooth and without the buildup of intermediate byproducts.

Some studies associate reactive intermediate deaminase A with metabolic disorders involving amino acid imbalances. Diseases such as hyperoxaluria and nonketotic hyperglycinemia show altered activities of proteins connected to RIDA indicating its potential role in these conditions. The connection between RIDA and other proteins like glycine decarboxylase suggests a broader impact on the intricate balance of amino acids in the human body.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.. Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex (PubMed : 30930054, PubMed : 8973653). Acts by bridging YTHDF2 and the ribonuclease P/MRP complex (PubMed : 30930054). RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif : cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage (PubMed : 30930054).
See full target information RIDA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 39:877-887 PubMed37750664

2023

Selenomethionine suppresses head and neck squamous cell carcinoma progression through TopBP1/ATR and TCAB1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Xiaodong Wei,Jiwu Li
View all publications
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Product promise

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