AB229656

Anti-Rif1 antibody - C-terminal

Name: Anti-Rif1 antibody - C-terminal (ab229656) | Abcam
Brand: Abcam Limited
SKU: AB229656
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RIF1 antibody. C-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Telomere-associated protein RIF1.

View Alternative Names

Telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, RIF1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (AB229656)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (AB229656)

Paraffin-embedded human cervical carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody - C-terminal (AB229656)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody - C-terminal (AB229656)

4% paraformaldehyde fixed heLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229656 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (AB229656)

Paraffin-embedded human breast carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-Rif1 antibody - C-terminal (AB229656)

Rif1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229656. Western blot was performed from the immunoprecipitate using ab229656. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : Control IgG IP instead of ab229656 in HeLa whole cell extract.

Lane 2 : ab229656 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-Rif1 antibody - C-terminal (ab229656)

Predicted band size: 274 kDa

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Supplier Data

Western blot - Anti-Rif1 antibody - C-terminal (AB229656)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Rif1 antibody - C-terminal (ab229656) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 274 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Telomere-associated protein RIF1. The exact immunogen used to generate this antibody is proprietary information.

Q5UIP0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rif1 also known as Rap1-interacting factor 1 is a protein that aids in DNA end protection and repair processes. The protein has a molecular mass of approximately 240 kDa and is predominantly expressed in the nucleus of eukaryotic cells. Rif1 interacts directly with telomeric DNA and regulates DNA-double strand break (DSB) repair. Its activity ensures genomic integrity by coordinating access to damaged DNA ends which is essential in the context of maintaining telomere length and chromosomal stability.

Biological function summary

Rif1 plays a central role in the DNA damage response and DNA replication. It operates as part of the shieldin complex which participates in non-homologous end joining (NHEJ) by protecting DNA ends from extensive resection. By doing so Rif1 directs the repair pathway choice towards NHEJ over homologous recombination enabling cell cycle progression in conditions of DNA stress. Its function is particularly significant during the S and G1 phases of the cell cycle during which Rif1 modulates replication timing and forks stalling recovery.

Pathways

Several critical cellular processes involve Rif1 particularly DNA damage repair and cell cycle regulation. Within these pathways Rif1 interacts with proteins such as 53BP1 which is instrumental in controlling pathway selectivity during DNA repair and it influences origin firing through its interplay with replication timing guards. Additionally Rif1 works in conjunction with ATM and ATR kinases which are key regulators in the phosphorylation cascade triggered by DNA damage. These interactions underpin Rif1’s pivotal role in cell cycle checkpoints and repair signaling pathways.

Aberrations in Rif1 function have links to cancer and premature aging diseases. Altered Rif1 expression or malfunction can contribute to tumorigenesis especially in cancers where DNA repair pathways are disrupted. This is due in part to its relationship with 53BP1 playing roles in tumor suppression pathways by maintaining genomic stability. Furthermore mutations affecting Rif1 are associated with conditions like Hutchinson-Gilford Progeria Syndrome. This connection to lamin A/C another protein involved in nuclear stability highlights its broader impact on maintaining cellular homeostasis and genome integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage : acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed : 15342490, PubMed : 28241136). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed : 23333306, PubMed : 28241136). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (PubMed : 28241136). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed : 23333306). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed : 23333306). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (By similarity). Promotes NHEJ of dysfunctional telomeres (By similarity).

See full target information Telomere-associated protein RIF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:1743-1760.e11 PubMed37116497

2023

Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo José Longarini,Helen Dauben,Carolina Locatelli,Anne R Wondisford,Rebecca Smith,Charlotte Muench,Andreas Kolvenbach,Michelle Lee Lynskey,Alexis Pope,Juan José Bonfiglio,Eva Pinto Jurado,Roberta Fajka-Boja,Thomas Colby,Marion Schuller,Ivan Ahel,Gyula Timinszky,Roderick J O'Sullivan,Sébastien Huet,Ivan Matic

Oncology reports 47: PubMed34738630

2021

Androgen deprivation‑induced OPHN1 amplification promotes castration‑resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junjiang Liu,Yunxia Zhang,Shoubin Li,Fuzhen Sun,Gang Wang,Dong Wei,Tao Yang,Shouyi Gu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Rif1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (AB229656)

Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody - C-terminal (AB229656)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (AB229656)

Immunoprecipitation - Anti-Rif1 antibody - C-terminal (AB229656)

Western blot - Anti-Rif1 antibody - C-terminal (AB229656)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise